IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Recombinant plant gamma Carbonic Anhydrase Homotrimers bind Inorganic Carbon.
Autor/es:
V. MARTIN; F. VILLARREAL; I. MIRAS; A. NAVAZA; A, HAOUZ; RM. GONZÁLEZ-LEBRERO; SB. KAUFMAN; E. ZABALETA
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2009 vol. 583 p. 3425 - 3430
ISSN:
0014-5793
Resumen:
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Gamma carbonic
anhydrases (gammaCA) are widespread in Prokaryotes. In Eukaryotes, homologous
genes were found only in plant genomes. In Arabidopsis and maize, the
corresponding gene products are subunits of mitochondrial Complex I. At
present, only gammaCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO2) hydration
activity. In the present work, it is shown that recombinant plant gammaCA2
could form homotrimers and bind H14CO3 . However, they are unable to catalyse
the reversible hydration of CO2. These results suggest that plant gammaCAs do
not act as carbonic anhydrases but with a related activity possibly
contributing to recycle CO2 in the context of photorespiration.