IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
The yeast endocytic protein Epsin-2 (Ent2) functions in a conserved cell division signaling pathway
Autor/es:
DEBARATI MUKHERJEE1*, BRIAN G. COON1*, DANIEL F. EDWARDS III1, CLAUDIA B. HANNA1,SILVIA A. LONGHI2, J. MICHAEL MCCAFFERY3, BEVERLY WENDLAND3, LILIA A. RETEGUI2 , ERFEI BI4 AND R. CLAUDIO AGUILAR1
Revista:
JOURNAL OF CELL SCIENCE
Editorial:
The Company of Biologists
Referencias:
Año: 2009 p. 2453 - 2463
ISSN:
0021-9533
Resumen:
The epsins are a family of adaptors involved in recruiting other endocytic proteins,
binding of ubiquitylated cargo and induction of membrane curvature. These molecules
bear a characteristic Epsin N-Terminal Homology (ENTH) domain and multiple peptide
motifs that mediate protein-protein interactions. We have previously demonstrated that
the ENTH domain of epsin is involved in Cdc42 signaling regulation. Here, we present
evidence that yeast epsin-2 (Ent2) plays a signaling role during cell division. We
observed that overexpression of the ENTH domain of Ent2 (ENTH2), but not Ent1,
promoted the formation of chains of cells and aberrant septa. This dominant-negative
effect resulted from ENTH2-mediated interference with septin assembly pathways. We
mapped the ENTH2 determinants responsible for induction of the phenotype and found
them to be important for efficient binding to the septin regulatory protein, Bem3.
Supporting the idea of a physiological role for epsin-2 in cell division, the protein
localized to sites of polarized growth and cytokinesis and was able to rescue a cell
division defect induced by Bem3 misregulation. Importantly, our data suggest that the
pathway affected by ENTH2 is conserved in mammals. Collectively, our findings provide
a potential molecular mechanism linking endocytosis (via epsin-2) with signaling
pathways regulating cell division.
pathways regulating cell division.
pathways regulating cell division.
pathways regulating cell division.
pathways regulating cell division.
pathways regulating cell division.
via epsin-2) with signaling
pathways regulating cell division.