IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity
Autor/es:
FAVALE NICOLAS; SANTACREU BRUNO; PESCIO LUCILA; MARQUEZ, MARÍA GABRIELA; STERIN-SPEZIALE NORMA B
Revista:
JLR PAPERS IN PRESS
Editorial:
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Referencias:
Lugar: Bethesda, Maryland; Año: 2015 vol. 2015 p. 786 - 800
ISSN:
0022-2275
Resumen:
Sphingolipids are relevant lipid components of eukaryotic cells. Besides regulating various cellular processes, sphingolipids provide the structural framework for plasma membrane organization. Particularly, sphingomyelin (SM) is associated with detergent-resistant microdomains. We have previously shown that the adherens junction (AJ) complex, the relevant cell-cell adhesion structure involved in cell differentiation and tissue organization, is located in a SM-rich membrane lipid domain. We have also demonstrated that under hypertonic conditions, MDCK cells acquire a differentiated phenotype with changes in sphingolipid metabolism. For these reasons, we decided to evaluate whether SM metabolism is involved in the acquisition of the differentiated phenotype of MDCK cells. We found that SM synthesis mediated by SM Synthase 1 (SMS1) is involved in hypertonicity-induced formation of mature AJs, necessary for correct epithelial cell differentiation. Inhibition of SM synthesis impaired the acquisition of mature AJs, evoking a disintegration-like process reflected by the dissipation of E-cadherin, β- and α-catenins from the AJ complex. As a consequence, MDCK cells did not develop the hypertonicity-induced differentiated epithelial cell phenotype.