IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Snake venomics of the South and Central American Bushmaster. Comparison of the toxin composition of Lachesiss muta gathered from proteomic versus transcriptomic analysis.
Autor/es:
LIBIA SANZ, JOSÉ ESCOLANO, MASSIMO FERRETTI, MIRTHA J. BISCOGLIO, ELENA RIVERA, ERNESTO J. CRESCENTI, YAMILETH ANGULO, BRUNO LOMONTE,JOSÉ MARÍA GUTIÉRREZ, JUAN J. CALVETE
Revista:
Otra revista
Editorial:
ELSEVIER
Referencias:
Lugar: España; Año: 2008 vol. 71 p. 46 - 60
Resumen:
We report the proteomic characterization of the venoms of two closely related pit vipers of 21 the genus Lachesis, L. muta (South American Bushmaster) and L. stenophrys (Centralthe genus Lachesis, L. muta (South American Bushmaster) and L. stenophrys (Central 22 American Bushmaster), and compare the toxin repertoire of the former revealed throughAmerican Bushmaster), and compare the toxin repertoire of the former revealed through 23 a proteomic versus a transcriptomic approach. The protein composition of the venoms ofa proteomic versus a transcriptomic approach. The protein composition of the venoms of 24 Lachesis muta and L. stenophrys were analyzed by RP-HPLC, N-terminal sequencing, MALDI-Lachesis muta and L. stenophrys were analyzed by RP-HPLC, N-terminal sequencing, MALDI- 25 TOF peptide mass fingerprinting and CID-MS/MS. Around 30–40 proteins of molecularTOF peptide mass fingerprinting and CID-MS/MS. Around 30–40 proteins of molecular 26 masses in the range of 13–110 kDa and belonging, respectively, to only 8 and 7 toxin familiesmasses in the range of 13–110 kDa and belonging, respectively, to only 8 and 7 toxin families 27 were identified in L. muta and L. stenophrys venoms. In addition, both venoms contained awere identified in L. muta and L. stenophrys venoms. In addition, both venoms contained a 28 large number of bradykinin-potentiating peptides (BPP) and a C-type natriuretic peptidelarge number of bradykinin-potentiating peptides (BPP) and a C-type natriuretic peptide 29 (C-NP). BPPs and C-NP comprised around 15% of the total venom proteins. In both species,(C-NP). BPPs and C-NP comprised around 15% of the total venom proteins. In both species, 30 themost abundant proteinswereZn2+-metalloproteinases (32–38%) and serine proteinasesthemost abundant proteinswereZn2+-metalloproteinases (32–38%) and serine proteinases 31 (25–31%), followed by PLA2s (9–12%), galactose-specific C-type lectin (4–8%), L-amino acid(25–31%), followed by PLA2s (9–12%), galactose-specific C-type lectin (4–8%), L-amino acid 32 oxidase (LAO, 3–5%), CRISP (1.8%; found in L. muta but not in L. stenophrys), and NGF (0.6%).oxidase (LAO, 3–5%), CRISP (1.8%; found in L. muta but not in L. stenophrys), and NGF (0.6%). 33 On the other hand, only six L. muta venom-secreted proteins matched any of the previouslyOn the other hand, only six L. muta venom-secreted proteins matched any of the previously 34 reported 11 partial or full-length venom gland transcripts, and venom proteome andreported 11 partial or full-length venom gland transcripts, and venom proteome and 35 transcriptome depart in their relative abundances of different toxin families. As expectedtranscriptome depart in their relative abundances of different toxin families. As expected 36 fromtheir close phylogenetic relationship, the venoms of L. muta and L. stenophrys share (orfromtheir close phylogenetic relationship, the venoms of L. muta and L. stenophrys share (or 37 contain highly similar) proteins, in particular BPPs, serine proteinases, a galactose-specificcontain highly similar) proteins, in particular BPPs, serine proteinases, a galactose-specific 38 C-type lectin, and LAO. However, they dramatically depart in their respective PLA2C-type lectin, and LAO. However, they dramatically depart in their respective PLA2 39 complement. Intraspecific quantitative and qualitative differences in the expression ofcomplement. Intraspecific quantitative and qualitative differences in the expression of 40 PLA2molecules were found when the venoms of five L. muta specimens (3 from Bolivia andPLA2molecules were found when the venoms of five L. muta specimens (3 from Bolivia and 41 2 from Peru) and the venom of the same species purchased from Sigma were compared. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. 2 from Peru) and the venom of the same species purchased from Sigma were compared. gene family, and suggests that functional differences due to structural changes in PLA2s2s 44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and 45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for 46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the 47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying 48 structure–function correlations of individual toxins.structure–function correlations of individual toxins. 42 These observations indicate that these class of toxins represents a rapidly-evolving These observations indicate that these class of toxins represents a rapidly-evolving