IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Snake venomics of the South and Central American Bushmaster. Comparison of the toxin composition of Lachesiss muta gathered from proteomic versus transcriptomic analysis.
Autor/es:
LIBIA SANZ, JOSÉ ESCOLANO, MASSIMO FERRETTI, MIRTHA J. BISCOGLIO, ELENA RIVERA, ERNESTO J. CRESCENTI, YAMILETH ANGULO, BRUNO LOMONTE,JOSÉ MARÍA GUTIÉRREZ, JUAN J. CALVETE
Revista:
Otra revista
Editorial:
ELSEVIER
Referencias:
Lugar: España; Año: 2008 vol. 71 p. 46 - 60
Resumen:
We report the proteomic characterization of the venoms of two closely related pit vipers of
21 the genus Lachesis, L. muta (South American Bushmaster) and L. stenophrys (Centralthe genus Lachesis, L. muta (South American Bushmaster) and L. stenophrys (Central
22 American Bushmaster), and compare the toxin repertoire of the former revealed throughAmerican Bushmaster), and compare the toxin repertoire of the former revealed through
23 a proteomic versus a transcriptomic approach. The protein composition of the venoms ofa proteomic versus a transcriptomic approach. The protein composition of the venoms of
24 Lachesis muta and L. stenophrys were analyzed by RP-HPLC, N-terminal sequencing, MALDI-Lachesis muta and L. stenophrys were analyzed by RP-HPLC, N-terminal sequencing, MALDI-
25 TOF peptide mass fingerprinting and CID-MS/MS. Around 3040 proteins of molecularTOF peptide mass fingerprinting and CID-MS/MS. Around 3040 proteins of molecular
26 masses in the range of 13110 kDa and belonging, respectively, to only 8 and 7 toxin familiesmasses in the range of 13110 kDa and belonging, respectively, to only 8 and 7 toxin families
27 were identified in L. muta and L. stenophrys venoms. In addition, both venoms contained awere identified in L. muta and L. stenophrys venoms. In addition, both venoms contained a
28 large number of bradykinin-potentiating peptides (BPP) and a C-type natriuretic peptidelarge number of bradykinin-potentiating peptides (BPP) and a C-type natriuretic peptide
29 (C-NP). BPPs and C-NP comprised around 15% of the total venom proteins. In both species,(C-NP). BPPs and C-NP comprised around 15% of the total venom proteins. In both species,
30 themost abundant proteinswereZn2+-metalloproteinases (3238%) and serine proteinasesthemost abundant proteinswereZn2+-metalloproteinases (3238%) and serine proteinases
31 (2531%), followed by PLA2s (912%), galactose-specific C-type lectin (48%), L-amino acid(2531%), followed by PLA2s (912%), galactose-specific C-type lectin (48%), L-amino acid
32 oxidase (LAO, 35%), CRISP (1.8%; found in L. muta but not in L. stenophrys), and NGF (0.6%).oxidase (LAO, 35%), CRISP (1.8%; found in L. muta but not in L. stenophrys), and NGF (0.6%).
33 On the other hand, only six L. muta venom-secreted proteins matched any of the previouslyOn the other hand, only six L. muta venom-secreted proteins matched any of the previously
34 reported 11 partial or full-length venom gland transcripts, and venom proteome andreported 11 partial or full-length venom gland transcripts, and venom proteome and
35 transcriptome depart in their relative abundances of different toxin families. As expectedtranscriptome depart in their relative abundances of different toxin families. As expected
36 fromtheir close phylogenetic relationship, the venoms of L. muta and L. stenophrys share (orfromtheir close phylogenetic relationship, the venoms of L. muta and L. stenophrys share (or
37 contain highly similar) proteins, in particular BPPs, serine proteinases, a galactose-specificcontain highly similar) proteins, in particular BPPs, serine proteinases, a galactose-specific
38 C-type lectin, and LAO. However, they dramatically depart in their respective PLA2C-type lectin, and LAO. However, they dramatically depart in their respective PLA2
39 complement. Intraspecific quantitative and qualitative differences in the expression ofcomplement. Intraspecific quantitative and qualitative differences in the expression of
40 PLA2molecules were found when the venoms of five L. muta specimens (3 from Bolivia andPLA2molecules were found when the venoms of five L. muta specimens (3 from Bolivia and
41 2 from Peru) and the venom of the same species purchased from Sigma were compared.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
2 from Peru) and the venom of the same species purchased from Sigma were compared.
gene family, and suggests that functional differences due to structural changes in PLA2s2s
44 molecules among these snakes may have been a hallmark during speciation andmolecules among these snakes may have been a hallmark during speciation and
45 adaptation of diverging snake populations to new ecological niches, or competition foradaptation of diverging snake populations to new ecological niches, or competition for
46 resources in existing ones. Our data may contribute to a deeper understanding of theresources in existing ones. Our data may contribute to a deeper understanding of the
47 biology and ecology of these snakes, and may also serve as a starting point for studyingbiology and ecology of these snakes, and may also serve as a starting point for studying
48 structurefunction correlations of individual toxins.structurefunction correlations of individual toxins.
42 These observations indicate that these class of toxins represents a rapidly-evolving These observations indicate that these class of toxins represents a rapidly-evolving