IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
EFFECT OF LIGANDS TO TOLL-LIKE RECEPTORS (TLR) 3, 7 AND 9 ON MICE INFECTED WITH MOUSE HEPATITIS VIRUS A59
Autor/es:
JOSÉ L. APARICIO ; M DUHALDE VEGA; L.A. RETEGUI
Revista:
Open Journal of Immunology
Editorial:
Irvine, CA : Scientific Research Publ.
Referencias:
Año: 2014 vol. 4 p. 129 - 138
ISSN:
2162-450X
Resumen:
Mice infected with mouse hepatitis virus A59 (MHV-A59), an enveloped, positive-strand RNA Coronavirus, induce hepatitis, thymus involution, IgG2a-restricted hypergammaglobulina-emia, transaminase release and autoantibodies (autoAb) to liver and kidney fumarylace-toacetate hydrolase (FAH). Since Toll-like receptors (TLR) play a central role in innate immunity, we explored the ef-fects of TLR3, 7 and 9 stimulation on MHV mouse infection. Thus, the animals were treated with Poly (I:C), Loxoribine and CpG, the respective TLR ligands. MHV-infected mice inoculated with Poly (I:C) had significant lower levels of plasma transa-minases and Ig, anti-MHV Ab, and uric acid than MHV-infected animals, whereas autoAb to kidney tissue were observed. Loxoribine only produced a slight decrease of uric acid levels and serum Ig. CpG showed deleterious effects on MHV-infected mice, since survival of ani-mals dramatically dropped to about 10%. AutoAb to murine tissues and uric acid release were not affected, whereas transaminases and anti-MHV Ab were slightly elevated. Besides, CpG administration produced a decrease of the high levels of serum Ig induced by the virus. Therefore, results indicated that TLR3 stimulation appeared to protect the animals against the viral infection, whereas CpG aggravated its signs. Loxoribine, the TLR7 ligand, did not show major effects.