IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Membrane lipid composition plays a cenytral role in the maintenance of the epithelial cell adhesion to the extracellular matrix
Autor/es:
MARÍA GABRIELA MARQUEZ; FRANCISCO LEOCATA NIETO; MARÍA C FERNANDEZ-TOME; NICOLÁS OCTAVIO FAVALE; NORMA STERIN-SPEZIALE
Revista:
LIPIDS
Editorial:
Springer
Referencias:
Año: 2007
ISSN:
0024-4201
Resumen:
Abstract Focal contacts (FC) are membrane-associatedFocal contacts (FC) are membrane-associated
11 multi-protein complexes that mediate cell-extracellularmulti-protein complexes that mediate cell-extracellular
12 matrix (ECM) adhesion. FC complexes are inserted inmatrix (ECM) adhesion. FC complexes are inserted in
13 detergent-resistant membrane microdomains enriched indetergent-resistant membrane microdomains enriched in
14 phosphatidylinositol-4, 5-bisphosphate (PtdIns(4,5)P2);phosphatidylinositol-4, 5-bisphosphate (PtdIns(4,5)P2);
15 however, the influence of membrane lipid composition inhowever, the influence of membrane lipid composition in
16 the preservation of FC structures has not been extensivelythe preservation of FC structures has not been extensively
17 addressed. In the present work, we studied the contributionaddressed. In the present work, we studied the contribution
18 of membrane lipids to the preservation of renal epithelialof membrane lipids to the preservation of renal epithelial
19 cell adhesion structures. We biochemically characterizedcell adhesion structures. We biochemically characterized
20 the lipid composition of membrane-containing FC com-the lipid composition of membrane-containing FC com-
21 plexes. By using cholesterol and PtdIns(4,5)P2 affectingplexes. By using cholesterol and PtdIns(4,5)P2 affecting
22 agents, we demonstrated that such agents did not affect anyagents, we demonstrated that such agents did not affect any
23 particular type of lipid but induced the formation of newparticular type of lipid but induced the formation of new
24 FC-containing domains of completely different lipidFC-containing domains of completely different lipid