IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Membrane lipid composition plays a cenytral role in the maintenance of the epithelial cell adhesion to the extracellular matrix
Autor/es:
MARÍA GABRIELA MARQUEZ; FRANCISCO LEOCATA NIETO; MARÍA C FERNANDEZ-TOME; NICOLÁS OCTAVIO FAVALE; NORMA STERIN-SPEZIALE
Revista:
LIPIDS
Editorial:
Springer
Referencias:
Año: 2007
ISSN:
0024-4201
Resumen:
Abstract Focal contacts (FC) are membrane-associatedFocal contacts (FC) are membrane-associated 11 multi-protein complexes that mediate cell-extracellularmulti-protein complexes that mediate cell-extracellular 12 matrix (ECM) adhesion. FC complexes are inserted inmatrix (ECM) adhesion. FC complexes are inserted in 13 detergent-resistant membrane microdomains enriched indetergent-resistant membrane microdomains enriched in 14 phosphatidylinositol-4, 5-bisphosphate (PtdIns(4,5)P2);phosphatidylinositol-4, 5-bisphosphate (PtdIns(4,5)P2); 15 however, the influence of membrane lipid composition inhowever, the influence of membrane lipid composition in 16 the preservation of FC structures has not been extensivelythe preservation of FC structures has not been extensively 17 addressed. In the present work, we studied the contributionaddressed. In the present work, we studied the contribution 18 of membrane lipids to the preservation of renal epithelialof membrane lipids to the preservation of renal epithelial 19 cell adhesion structures. We biochemically characterizedcell adhesion structures. We biochemically characterized 20 the lipid composition of membrane-containing FC com-the lipid composition of membrane-containing FC com- 21 plexes. By using cholesterol and PtdIns(4,5)P2 affectingplexes. By using cholesterol and PtdIns(4,5)P2 affecting 22 agents, we demonstrated that such agents did not affect anyagents, we demonstrated that such agents did not affect any 23 particular type of lipid but induced the formation of newparticular type of lipid but induced the formation of new 24 FC-containing domains of completely different lipidFC-containing domains of completely different lipid