IQUIFIB 02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Behaviour of the antioxidant defence system and heme oxygenase-1 protein expression in fructose-hypertensive rats
Autor/es:
ARIEL H. POLIZIO; SOLEDAD GONZALES; MARINA C. MUÑOZ; CLARA PEÑA; MARIA L. TOMARO
Revista:
Clinical and Experimental Pharmacology and Physiology
Editorial:
Blackwell Publishing Asia Pty Ltd
Referencias:
Año: 2006 vol. 33 p. 734 - 739
Resumen:
SUMMARY
1. Addition of fructose to a rat diet for various periods of time
leads to hypertension, hyperinsulinaemia and dyslipidaemia and
provides a model for testing oxidative stress parameters in the
animals.
2. In the present study, oxidative stress generation, the soluble
and enzymatic defence system and heme oxygenase-1 (HO-1)
protein expression were investigated in the heart, liver and kidney
of rats fed fructose for a period of 1 or 8 months.
3. Compared with the control group, fructose-hypertensive
rats showed increased in lipid peroxidation only in the heart
after both 1 and 8 months of fructose treatment. Changes in the
behaviour of the soluble and enzymatic defence system and
HO-1 protein expression were different depending on the organ.
Increased or unaltered activities of anti-oxidant enzymes were
found in the liver and kidney, respectively. Induction of HO-1
prevented the generation of oxidative stress in the liver, where
the activity of anti-oxidant defence enzymes was not reduced.
Increased expression of HO-1 protein was not able to prevent
the generation of oxidative stress in the heart, where fructose
treatment diminished the activity of anti-oxidant enzymes.
4. The results of the present study demonstrate that upregulation
of HO-1 may prevent the generation of oxidative stress
only when the anti-oxidant defence system is still operative.
Key words: anti-oxidant enzymes, fructose hypertension,
heme oxygenase, oxidative stress, rats.
1. Addition of fructose to a rat diet for various periods of time
leads to hypertension, hyperinsulinaemia and dyslipidaemia and
provides a model for testing oxidative stress parameters in the
animals.
2. In the present study, oxidative stress generation, the soluble
and enzymatic defence system and heme oxygenase-1 (HO-1)
protein expression were investigated in the heart, liver and kidney
of rats fed fructose for a period of 1 or 8 months.
3. Compared with the control group, fructose-hypertensive
rats showed increased in lipid peroxidation only in the heart
after both 1 and 8 months of fructose treatment. Changes in the
behaviour of the soluble and enzymatic defence system and
HO-1 protein expression were different depending on the organ.
Increased or unaltered activities of anti-oxidant enzymes were
found in the liver and kidney, respectively. Induction of HO-1
prevented the generation of oxidative stress in the liver, where
the activity of anti-oxidant defence enzymes was not reduced.
Increased expression of HO-1 protein was not able to prevent
the generation of oxidative stress in the heart, where fructose
treatment diminished the activity of anti-oxidant enzymes.
4. The results of the present study demonstrate that upregulation
of HO-1 may prevent the generation of oxidative stress
only when the anti-oxidant defence system is still operative.
Key words: anti-oxidant enzymes, fructose hypertension,
heme oxygenase, oxidative stress, rats.
