Reactive oxygen species formation and cell death in catalase-deficient tobacco leaf discs exposed to paraquat

IANNONE MF; ROSALES EP; GROPPA MD; BENAVIDES MP

BIOLOGICAL TRACE ELEMENT RESEARCH

HUMANA PRESS INC

Año: 2012 vol. 146 p. 246 - 255

0163-4984

In the present work, the response of tobacco (Nicotiana tabaccum L.) wild type SR1 and transgenic CAT1AS plants (with a basal reduced CAT activity) was evaluated after exposure to the herbicide paraquat (PQ). Superoxide anion (O2.-) formation was inhibited at 3 h or 21 h of exposure, but H2O2 production and ion leakage increased significantly, both in SR1 or CAT1AS leaf discs. NADPH oxidase activity was constitutively 57% lower in non-treated transgenic leaves than in SR1 leaves and was greatly reduced both at 3 h or 21 h of PQ treatment. Superoxide dismutase (SOD) activity was significantly reduced by PQ at 21 h, showing a decrease from 70% to 55%, whereas catalase (CAT) activity decrease an average of 50% at 3 h of treatment and 90% at 21 h, in SR1 and CAT1AS, respectively. Concomitantly, total CAT protein content was shown to be reduced in non-treated CAT1AS plants compared to control SR1 leaf discs at both exposure times. PQ decreased CAT expression in SR1 or CAT1AS plants at 3 h and 21 h of treatment. The mechanisms underlying PQ-induced cell death were not exclusively related to ROS formation and oxidative stress in tobacco wild type or transgenic CAT-deficient plants.