Comparative oxidative stress in subantarctic invertebrates.

ANALÍA PEREZ; GABRIELA MALANGA; CAROLINA ROMERO; GUSTAVO ALEJANDRO LOVRICH; SUSANA PUNTARULO

Bahía Blanca, Argentina

Simposio; Symposium: Science and management of estuaries and coasts: A tale of two hemispheres.; 2008

ECSA 44

Changes in the production of reactive oxygen species (ROS) on aquatic organisms had been related to environment conditions or physiological change. This study analyzed invertebrate enzymatic and non-enzymatic antioxidant, and lipid peroxidation under physiological conditions. The species considered from the Beagle Channel were: limpets Nacella deaurata and N. magellanica, sea urchin Loxechinus albus, and stone crab Paralomis granulosa. Tissues analyzed were digestive gland, gut and hepatopancreas for limpets, sea urchins and stone crabs, respectively. Cellular antioxidant status was measured by catalase (CAT) and superoxide dismutase (SOD) activity, a-tocopherol and b-carotene content. Furthermore, the lipid peroxidation was measured by quantification of TBARS. The CAT activity was 2.3±0.2 and 1.7±0.2 U/mg protein, whereas the SOD activity was 3.2±0.6 and 1.4±0.2 U/mg protein, for N. magellanica and N. deaurata, respectively. Non-enzymatic antioxidants were significantly lower in N. magellanica as compared to N. deaurata. Moreover, Paralomis granulosa showed values of CAT and SOD activity of 46±16 nmol/mg protein and 4±1 U SOD/mg protein, respectively. The content of TBARS in N. deaurata was 23 nmol/mgWW and was significantly higher than that found in N. magellanica. Sea urchins presented values of TBARS of 13.5 nmol/mgWW, whereas the value for Paralomis granulosa was 32.0±8.1 CHP eq/mg WW. In addition, values of a-tocopherol and b-carotene content in sea urchin were 0.12 pmol/mgFW and 0.08 pmol/mgFW, respectively. All species showed antioxidant capacity in the tissues analyzed, probably related to a routine exposition to changes environmental.