MACNBR   00242
MUSEO ARGENTINO DE CIENCIAS NATURALES "BERNARDINO RIVADAVIA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Tyrosine hydroxylase in the central nervous system of Apidae and Triatominae
Autor/es:
GALVANI GERÓNIMO LUIS; SETTEMBRINI BEATRIZ P; LILIÁN CANAVOSO; JIMENA LEYRIA
Lugar:
Orlando
Reunión:
Congreso; 2016 International Congress of Entomology; 2016
Institución organizadora:
Entomological Society of America
Resumen:
Tyrosine hydroxylase (TH) catalyzes the rate limiting step of cathecolamine biosynthesis. In insects, cathecholamines are involved in locomotion, learning and cuticle sclerotization among other functions. Two isoforms of TH have been reported; the shorter one which lacks a highly acidic region is present in neural cells.The distribution of TH was studied in the nervous system of triatomine bugs (Triatoma infestans) and bees (Melitoma segmentaria, Thygater analis and Leiopodus lacertinus) using our standardized immunocytochemistry protocol. The TH antiserum developed by O? Gorman et al. (2007) in Manduca sexta was employed in the immunolocalization of cell bodies as well as in western blot assays.The TH antiserum allowed the detection of immunoreactive (IR) somata and neurites in the brain of all the species analyzed. Most of the immunopositive cell bodies displayed a uniform size of about 10 µm. In the protocerebrum, clusters with variable numbers of somata were observed above the mushroom body calices, in the pars intercerebralis as well as around the central complex. The antennal lobe glomeruli and the dorsal lobe also housed immunoreactive elements in all these species. It was found that within the bee species, only L. lacertinus contained TH-IR cell bodies in the proximal optic lobe, a finding also shared by the triatomine T.infestans.The anti-TH antiserum recognized an immunoreactive band of ~60kDa, compatible with a predicted mass for the monomer form of 66.3 kDa. The presence of TH in the same brain areas of insect species from separate orders suggests highly conserved functions for catecholamines.