MACNBR   00242
MUSEO ARGENTINO DE CIENCIAS NATURALES "BERNARDINO RIVADAVIA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The use of DNA barcodes to assess the biodiversity patterns of megadiverse groups in tropical regions: The PANCODING project.
Autor/es:
FACUNDO MARTÍN LABARQUE; LUIS NORBERTO PIACENTINI; MARTÍN JAVIER RAMÍREZ; GUSTAVO HORMIGA; DIMITAR DIMITROV; LIGIA BENAVIDES; MIGUEL ÁNGEL ARNEDO; JOAN PONS
Reunión:
Workshop; Extending and Enhancing DNA Barcoding Research in Argentina and Neighboring Countries, Second Leading Labs Training Workshop; 2010
Institución organizadora:
Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"
Resumen:
The main goal of the PANCODING project is to evaluate the advantages of the DNA barcoding techniques to estimate patterns of species-richness and turnover of a megadiverse group (spiders) in a biodiversity hot-spot (the montane forests of Panama).   We conducted a semi-quantitative sampling of four one hectare plots along a longitudinal transect spanning 350 km on the main cordillera of Panama. We have sorted more than 30,000specimens, 60% of which were immature, nearly 25% females and 15% males. Of the 408 hypothesized morphospecies, 25% were represented by singletons and 10% by doubletons in our samples. These percentages fall within values reported from previous biodiversity inventories of Arthropods in Neotropical and Palearctic region. We use these data to estimate alfa and beta diversity using species acumulation curves and non-parametric estimators (ICE, ACE, Chao1, Chao2, Jackknife 1, Jackknife 2), and complementarity and similarity estimators. Results indicate levels of inventory completion of 70% for each locality and 10% of complementarity between localities.   In parallel, we have sequenced the first half of the cox1 mitochondrial gene of more than 2,400 specimens, including immatures, which yielded more than 1500 unique haplotypes. Distinct genetic lineages were identified using the GMYC method.   Here we present and contrast the results of comparing the patterns of genetic based alfa and beta diversitity with those inferred from morphological identification. In addition, we will discuss the effect of including DNA-aided identified immatures, generally removed from morphology-based analyses, on the estimates of alfa and beta diversity.