MUSEO ARGENTINO DE CIENCIAS NATURALES "BERNARDINO RIVADAVIA"
Unidad Ejecutora - UE
A new minimally-invasive and inexpensive sampling method for genetic studies in pinnipeds
IBAÑEZ, EZEQUIEL A.; TÚNEZ, JUAN I.; PERALTA, DIEGO M.; CAPPOZZO, HUMBERTO L.; LUCERO, SERGIO
Springer Berlin Heidelberg
Lugar: Berlin; Año: 2019 vol. 65 p. 11 - 11
The choice of sampling method is essential in the development of a population genetic study. This choice influences the type of samples that can be obtained and, therefore, the quality of the extracted DNA. Sampling methods can be classified into invasive, non-invasive and minimally invasive. This last category is preferred, as it minimizes handling, damage and the degree of disturbance to animals, while still providing enough DNA for genetic studies. Here, we describe a very low cost and easy to manufacture minimally invasive sampler, developed to obtain hair samples from pinnipeds. The sampler was highly effective both at reaching animals and collecting a good number of hairs. We compared the efficiency of hair samples obtained using our device, versus tissue samples collected from dead animals, by counting successful mtDNA amplifications and sequencing. Hair samples amplified more efficiently compared to tissue samples, but sequencing was equally efficient for both sample types. In conclusion, the sampler described is an excellent option to obtain DNA for genetic studies. This minimally invasive technique can also be used to sample hair for other studies, such as estimation of environmental contaminants, measurement of individual hormone levels or diet determination through the analysis of stable isotopes, among other potential uses.