MUSEO ARGENTINO DE CIENCIAS NATURALES "BERNARDINO RIVADAVIA"
Unidad Ejecutora - UE
Distribution and characterization of nitric oxide synthase in the nervous system of Triatoma infestans (Insecta: Heteroptera)
SETTEMBRINI BEATRIZ P; CORONEL MARÍA F; NOWICKI SUSANA; NIGHORN ALAN J; VILLAR MARCELO J
CELL AND TISSUE RESEARCH
Lugar: Heidelberg; Año: 2007 vol. 328 p. 421 - 421
The biochemical characterization of nitric oxide synthase and its distribution in the CNS were studied in the heteropteran bug Triatoma infestans. NOS-like immunoreactivity was found in the brain, subesophageal ganglion and thoracic ganglia by using high sentitivity immunocytochemistry. Western Blotting showed that a universal NOS antiserum recognized a band at 134 kDa which matches the expected size of the protein. Analiysis of the kinetics of nitric oxide production revealed a fully active enzyme in tissue samples of this heteropteran insect