Karyotypic Data on 28 Species of Scinax (Amphibia, Anura, Hylidae): Diversity and Informative Variation

CARDOZO, D.; LEME, D.M.; BORTOLETO, J.F.; CATROLI, G.F.; BALDO, D; FAIVOVICH, J.; KOLENC, F.; SILVA, A.P.Z.; BORTEIRO, C.; HADDAD, C. F. B.; KASAHARA, S.

COPEIA

AMER SOC ICHTHYOLOGISTS HERPETOLOGISTS

Año: 2011 vol. 2011 p. 251 - 263

0045-8511

<!-- /* Font Definitions */ @font-face {font-family:"Times New Roman"; panose-1:0 2 2 6 3 5 4 5 2 3; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:50331648 0 0 0 1 0;} @font-face {font-family:TimesNewRoman; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"Times New Roman"; mso-font-charset:77; mso-generic-font-family:roman; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:50331648 0 0 0 1 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0in; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman";} table.MsoNormalTable {mso-style-parent:""; font-size:10.0pt; font-family:"Times New Roman";} @page Section1 {size:595.0pt 842.0pt; margin:1.0in 1.25in 1.0in 1.25in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> The hylid frog genus Scinax is the most species-rich within Hylinae, with more than 90 recognized species, grouped in the Scinax catharinae and Scinax ruber clades. Cytogenetic information about Scinax is scarce, with data available for only 19 species, including NORs position in five of them. The karyotypes of 14 species of the Scinax catharinae clade and 14 of the S. ruber clade were analyzed, several of them for the first time. All the analyzed species presented 2n=2x=24 biarmed chromosomes (FN=48), without identifiable sex chromosomes, and relatively similar karyotypes. There are three states associated with the morphology of pair 1, each corresponding to the S. catharinae and S. perpusillus groups, both within the S. catharinae clade, and the third to the S. ruber clade. The size of chromosome pair 1 and the morphology of pairs 2, 3, 4, 6, and 7 also differentiate the species of both major clades. Species of the S. ruber clade in general have NORs in pair 11, as is commonly observed among hylines with 2n=24. The position in the long arms of pair 11 is interstitial in S. fuscomarginatus, S. fuscovarius, S. nasicus, S. similis, S. squalirostris, and S. uruguayus, and terminal in S. acuminatus, S. curicica, S. duartei, S. granulatus, S. hayii, and S. perereca. The single exception among species of the S. ruber clade is S. alter, that has terminal Ag-NORs at the long arms of pair 3 which may be autapomorphic. Most species of the S. catharinae clade have NORs in pair 6, corresponding to a putative synapomorphy of this clade, while the NORs in pair 11 that occurs in S. canastrensis is most parsimoniously interpreted as a reversion. C-banding is predominantly centromeric, but in the S. catharinae clade there is a greater amount of heterochromatin than in the S. ruber clade. This study corroborates the occurrence of informative variation, some already considered in a previous cladistic analysis, and reports new characters, outlining the significance of cytogenetic data for the systematics of Scinax.