Distribution of flavonoids in Lotus tenuis populations in grazed grasslands

MENDOZA RODOLFO; GARCÍA ILEANA; FERRARO GRACIELA; FILIP ROSANA

LOTUS NEWSLETTER

INSTITUTO NACIONAL DE INVESTIGACION AGROPECUARIA

Lugar: Colonia; Año: 2011 vol. 41

1510-7809

Lotus tenuis Waldst. et Kit. (=Lotus glaber Mill.) is a widely spread leguminous plant which grows naturally in grasslands of the .Flooding Pampas. of Argentina. It is highlyWaldst. et Kit. (=Lotus glaber Mill.) is a widely spread leguminous plant which grows naturally in grasslands of the .Flooding Pampas. of Argentina. It is highly.Flooding Pampas. of Argentina. It is highly appreciated by farmers because it is a good source of nutritive forage for beef and dairy cattle in nutrient deficient soils. In addition to its plasticity, one of the hypotheses to explain the successful spread of L. tenuis along the last 30 years in the Flooding Pampas is the ecotypes differentiation. The aim of the present work was to investigate whether the presence of different and specific flavonoids content in L. tenuis leaf tissue are associated with populations which grown in specific soil conditions and may constitute an additional means of distinguishing ecotypes. In our previous work, individuals of L. tenuis from populations growing in soils of different location and chemical characteristics were collected, cultivated in glasshouse and their shoots compared by their flavonoids profile. The flavonoids detected were differed among locations in both, quantity and quality. In the present work, individuals of L. tenuis were collected from a same lot of land along a transect of 0.51 km at four sites separated by both longitude and altitude. Differences in altitude determined differences in soil chemical characteristics. After collection at field site, the plants were analysed by their flavonoids in shoot tissue with no previous cultivation in glasshouse as we did in the previous work. Three different flavonoids were detected at the four sampled sites: isorhamnetin, kaempferol 7-methyl ether and quercetin-7-methyl ether. The L. tenuis individuals, collected from a saline-sodic site, the lowest in altitude and poor in terms of soil fertility for plant growth showed in both work, glasshouse cultivation and field condition, a distinctly different qualitative and quantitative profile with respect to all other samples suggesting an ecotype differentiation.L. tenuis along the last 30 years in the Flooding Pampas is the ecotypes differentiation. The aim of the present work was to investigate whether the presence of different and specific flavonoids content in L. tenuis leaf tissue are associated with populations which grown in specific soil conditions and may constitute an additional means of distinguishing ecotypes. In our previous work, individuals of L. tenuis from populations growing in soils of different location and chemical characteristics were collected, cultivated in glasshouse and their shoots compared by their flavonoids profile. The flavonoids detected were differed among locations in both, quantity and quality. In the present work, individuals of L. tenuis were collected from a same lot of land along a transect of 0.51 km at four sites separated by both longitude and altitude. Differences in altitude determined differences in soil chemical characteristics. After collection at field site, the plants were analysed by their flavonoids in shoot tissue with no previous cultivation in glasshouse as we did in the previous work. Three different flavonoids were detected at the four sampled sites: isorhamnetin, kaempferol 7-methyl ether and quercetin-7-methyl ether. The L. tenuis individuals, collected from a saline-sodic site, the lowest in altitude and poor in terms of soil fertility for plant growth showed in both work, glasshouse cultivation and field condition, a distinctly different qualitative and quantitative profile with respect to all other samples suggesting an ecotype differentiation.L. tenuis leaf tissue are associated with populations which grown in specific soil conditions and may constitute an additional means of distinguishing ecotypes. In our previous work, individuals of L. tenuis from populations growing in soils of different location and chemical characteristics were collected, cultivated in glasshouse and their shoots compared by their flavonoids profile. The flavonoids detected were differed among locations in both, quantity and quality. In the present work, individuals of L. tenuis were collected from a same lot of land along a transect of 0.51 km at four sites separated by both longitude and altitude. Differences in altitude determined differences in soil chemical characteristics. After collection at field site, the plants were analysed by their flavonoids in shoot tissue with no previous cultivation in glasshouse as we did in the previous work. Three different flavonoids were detected at the four sampled sites: isorhamnetin, kaempferol 7-methyl ether and quercetin-7-methyl ether. The L. tenuis individuals, collected from a saline-sodic site, the lowest in altitude and poor in terms of soil fertility for plant growth showed in both work, glasshouse cultivation and field condition, a distinctly different qualitative and quantitative profile with respect to all other samples suggesting an ecotype differentiation.L. tenuis from populations growing in soils of different location and chemical characteristics were collected, cultivated in glasshouse and their shoots compared by their flavonoids profile. The flavonoids detected were differed among locations in both, quantity and quality. In the present work, individuals of L. tenuis were collected from a same lot of land along a transect of 0.51 km at four sites separated by both longitude and altitude. Differences in altitude determined differences in soil chemical characteristics. After collection at field site, the plants were analysed by their flavonoids in shoot tissue with no previous cultivation in glasshouse as we did in the previous work. Three different flavonoids were detected at the four sampled sites: isorhamnetin, kaempferol 7-methyl ether and quercetin-7-methyl ether. The L. tenuis individuals, collected from a saline-sodic site, the lowest in altitude and poor in terms of soil fertility for plant growth showed in both work, glasshouse cultivation and field condition, a distinctly different qualitative and quantitative profile with respect to all other samples suggesting an ecotype differentiation.L. tenuis were collected from a same lot of land along a transect of 0.51 km at four sites separated by both longitude and altitude. Differences in altitude determined differences in soil chemical characteristics. After collection at field site, the plants were analysed by their flavonoids in shoot tissue with no previous cultivation in glasshouse as we did in the previous work. Three different flavonoids were detected at the four sampled sites: isorhamnetin, kaempferol 7-methyl ether and quercetin-7-methyl ether. The L. tenuis individuals, collected from a saline-sodic site, the lowest in altitude and poor in terms of soil fertility for plant growth showed in both work, glasshouse cultivation and field condition, a distinctly different qualitative and quantitative profile with respect to all other samples suggesting an ecotype differentiation.L. tenuis individuals, collected from a saline-sodic site, the lowest in altitude and poor in terms of soil fertility for plant growth showed in both work, glasshouse cultivation and field condition, a distinctly different qualitative and quantitative profile with respect to all other samples suggesting an ecotype differentiation.