INVESTIGADORES
SCHILRREFF Priscila
congresos y reuniones científicas
Título:
NANO-Controlled Released System (NANOCRS) against Toxoplasma gondii for oral administration.
Autor/es:
PRIETO MJ, SCHILRREFFP, MORILLA MJ, ROMERO EL.
Lugar:
Mar del Plata, Buenos Aires
Reunión:
Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB); 2007
Resumen:
BT-P34. NANO-CONTROLLED RELEASED SYSTEM (NANOCRS) AGAINST TOXOPLASMA GONDII FOR ORALADMINISTRATION Prieto MJ, SchilrreffP, Morilla MJ, Romero EL. Universidad Nacional de Quilmes, Laboratorio de Diseño de Estrategias de Targeting de Drogas. E-mail: jprieto@unq.edu.ar Immunosuppressed patients in which reactivation of latent T.gondii infection is often fatal, are conventionally treated with high doses of sulfadiazine (SDZ) together with pyrimetamine, a drug of high toxicity. Dendrimers (D) exhibit unique properties that make them suitable candidates as nanoCRS. As part of our search for a nanoCRS capable of entering the host cells in order to increase the efficiency of SDZ delivery,we have previously shown thatDcan be used as nanoCRS of SDZ; complexes D-SDZ presented high antiparasitic activity on infected cells with T. gondii even at ultralow nanomolar doses. In this work we performed two sets of studies: in vitro set of studies was aimed to determine the D-SDZ membrane damage, and the intracellular uptake of D-SDZ labelled with FITC, onVero (endocytosis) and J774 (phagocytosis) cells; in vivo studies consisted of determining biodistribution and pharmacokinetic parameters of free and D-SDZ after i.v. and oral administration to healthy rats. Our results indicated that D-SDZ behaved strongly different from free SDZ, resulting in a high retention of the SDZ in blood circulation upon both administrations. Taken together those in vivo data with the high in vitro activity, we could expect that further administration of D-SDZ to infected animals could be employed to reduce therapeutic SDZ doses, avoiding the use of the highly toxic pyrimetamine. Posters BIOCELL 140 31 (Suppl.), 2007