3D IN VITRO TISSUE MODELS IN NANOMEDICINE RESEARCH

PRISCILA SCHILRREFF; MORILLA MARIA JOSE; EDER ROMERO

Congreso; SAIC.SAI.SAFE 2016; 2016

With the increasing numbers of new nanomedicines there is a need to investigate their possible health adverse effects in humans. Nanomedicines can potentially be administered through a number of routes, including oral, dermal, pulmonar and ocular. Extensive in vitro and in vivo research is needed before nanomedicines will be suitable for medicinal use. For that purpose, millions of animals are used. However, 95 percent of new drugs fail because they do not work in humans or are unsafe, despite previously appearing safe in preclinical animal tests. According to US FDA, adverse drug reactions cause over 100.000 deaths annually. Besides, being costly and poor predictive of human toxicity due to inter-species differences, animal tests can be cruel and painful. Therefore, the replacement of animals with in vitro human cell-based models is required. In this context, 3D in vitro models provides a cellular microenvironment that preserves cell-cell interactions, function and tissue architecture and could be a useful tool for predicting the effects of nanomedicines in humans.A number of celular 3D models have been developed including skin, liver, cardiac, pulmonar, corneal and ephithelial intestinal tissues. This presentation will focus on technical specifications for the development of intestinal, normal and disease skin models used for the evaluation of toxicity, penetration and efficiency of new nanomedicines.