INVESTIGADORES
HERKOVITS Jorge
artículos
Título:
Lethal and teratogenic effects of naringenin evaluated by mean of an amphibian embryo toxicity test (AMPHITOX)"
Autor/es:
PEREZ-COLL C.S. AND HERKOVITS J.
Revista:
FOOD AND CHEMICAL TOXICOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2004 vol. 42 p. 299 - 306
ISSN:
0278-6915
Resumen:
The effect of naringenin on the survival and morphogenesis of amphibian
embryos was evaluated by means of the AMPHITOX test reporting early life stage
and chronic toxicity effects. Lethality, malformation incidence and the degree
of adverse effects were concentration-dependent. The Teratogenic Index (TI) for
naringenin was 2 pointing out the high developmental hazard of this substance.
For instance, 10 mg/l naringenin exerted 100% malformations while only 30% of
the abnormal embryos died. Main abnormalities were reduced body size, axial
curves, microcephaly, abdominal edema, underdeveloped gills and delayed
development. Scanning electronic microscopy (SEM) showed alterations in
epithelial cell shapes related to malformations. The results obtained by means
of treatment of Bufo arenarum embryos with naringenin from complete operculum
stage onwards show that at this final stage of development, the susceptibility
of the embryos to this flavonoid is slightly lower for lethal effects but
exerted sublethal adverse effects such as transient narcosis, abnormal
contortions, loss of equilibrium, reduced motility and edema. Overall, amphibian
early life stages appeared more susceptible to the embryotoxicity associated
with exposure to naringenin, especially at concentrations greater than 5 mg/l.
This increased susceptibility may result from the relatively high rates of
cellular differentiation and morphogenesis that occur at this early stage of
development.