INVESTIGADORES
HERKOVITS Jorge
artículos
Título:
Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae.
Autor/es:
ARONZON, CM; SANDOVAL, MT; HERKOVITS, J; PÉREZCOLL, CS
Revista:
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Editorial:
SETAC PRESS
Referencias:
Año: 2011 vol. 30 p. 2771 - 2777
ISSN:
0730-7268
Resumen:
Abstract—Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum wasevaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50,and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages wereplotted from 24 to 168 h, resulting from S.4 in a 24-h LC50 of 137 mg Cu2þ/L and a 168-h LC50 of 19.5mg Cu2þ/L. This result was inagreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period,between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showedno significant differences along exposure time (168-h LC50¼51mg Cu2þ/L), but in the case of S.28 toxicity increased slightly from a24-h LC50 of 138.6 mg Cu2þ/L to a 168-h LC50 of 104 mg Cu2þ/L, pointing out that, although the larval period was significantly moreresistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximatelytwo, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of orunderdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mec