INVESTIGADORES
ALONSO Silvia Del Valle
artículos
Título:
Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells
Autor/es:
SPERONI L, GASPARRI J, BUSTUOABAD V DE LOS A, CHIARAMONI NS, SMAGUR A, SZALA S, TAIRA MC, ALONSO S DEL V.
Revista:
ACTA BIOCHIMICA POLONICA
Editorial:
ACTA BIOCHIMICA POLONICA
Referencias:
Lugar: Warszawa, Poland ; Año: 2009 vol. 56 p. 249 - 253
ISSN:
0001-527X
Resumen:
Murine melanoma B16FO cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) lipo- somes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ra- tio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expres- sion had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.
