A model based in the radius of vesicles to predict the number of unilamellar liposomes

JORGE AM MONTANARI, PAULA L. BUCCI, SILVIA DELV. ALONSO

International Journal of Research in Pharmacy and Chemistry

IJRPC

Lugar: USA; Año: 2014 vol. 4 p. 484 - 489

In  particulate  systems  such  as  liposomes,  concentration  units  are  not  enough  to describe  the  drug  distribution,  as  suspensions  are  not  homogeneous.  In  certain  in  vitro assays, exposure to different number of particles introduces an extra variable regarding to contact phenomena. The aim is to achieve a rapid estimation of the number of unilamellar liposomes in a suspension. A simple mathematical method was developed; variables were the area and molecular weight of  lipids, and  the mean size of  the  liposomes. Unilamellar liposomes were prepared. Size was determined by dynamic  light scattering, and then the number of particles were determined by tunable resistive pulse sensing. There was about a 90% coincidence between the theoretical results and the number of counted liposomes. This model  could be useful  for  interpretation of  in  vitro experiments, when  results  could depend on the distribution of actives into different quantities of liposomes