INVESTIGADORES
ALONSO Silvia Del Valle
artículos
Título:
"Relationship between the adjuvant and cytotoxic effects of the positive charges and polymerization in liposomes".
Autor/es:
GASPARRI, JULIETA; SPERONI, LUCÍA; CHIARAMONI, NADIA SILVIA; ALONSO, SILVIA DEL VALLE.
Revista:
JOURNAL OF LIPOSOME RESEARCH
Editorial:
TAYLOR & FRANCIS INC
Referencias:
Lugar: Londres; Año: 2011 vol. 21 p. 124 - 133
ISSN:
0898-2104
Resumen:
AbstractVaccine development today encounters a main
obstacle, which is the need for effective adjuvants suitable for
clinical trials. Aluminum salts, discovered 70 years ago and, very
recently, MF59, are the only types of adjuvants currently used in
vaccines licensed by the U.S. Food and Drug Administration. Liposomes
represent an alternative approach to vaccine adjuvants. In this article,
we describe the inflammatory response and biological effect of
polymerization and the addition of positive charges in liposome
formulations. Nonpolymerized cationic (NP(+)) liposomes significantly
reduce metabolism in Vero cells after 24 hours. Correspondingly, both
NP(+) and polymerized cationic (P(+)) liposomes reduce cell viability
following a 48-hour incubation. Similar results were obtained with cells
from the peritoneal cavities of mice. Paradoxically, those liposomes
that presented clearly cytostatic or cytotoxic effects in vitro
stimulated metabolism and had a mitogenic effect in vivo. Finally, the
adjuvant effect was tested by immunization in BALB/c mice. The major
effect was obtained with NP(+) liposomes. Accordingly, we also
demonstrated that NP(+) liposomes injected into the dermis produced an
outstanding inflammatory reaction, showing the histopathological
characteristics of an inoculation granuloma. Thus, positive charge would
play an important role in the immunoadjuvant effect of liposomes by
conferring them cytotoxic capacity