CCT BAHIA BLANCA   20444
CENTRO CIENTIFICO TECNOLOGICO CONICET - BAHIA BLANCA
Centro Científico Tecnológico - CCT
congresos y reuniones científicas
Título:
AMPEROMETRIC bIOSENSOR FOR GLUCOSE DETERMINATION IN APPLE JUICE
Autor/es:
RISTOFF MARÍA E.,SAIDMAN SILVANA B., SORRIVAS VIVIANA, AND LOZANO JORGE E.
Lugar:
Stellenbosch
Reunión:
Congreso; VI International Technical Symposium on ¨Innovating the food value chain ¨ postharvest technology and agri-food processing; 2012
Resumen:
Abstract. Research on amperometric biosensors attracts lots of current interest beca use of their potential applications in the food industry. The objective of the present work was: (i) to assemble an amperometric biosensor based on glucose oxidase (GOx); (ji) to identify and eliminate (or reduce) interferences of electro-active compounds found in apple juice; and (ii), to compare glucose determination with both an standard biochemical method and the biosensor developed in this work. Carbon paste biosensors were prepared by hand-mixing glucose oxidase, graphite powder, paraffin, and ferrocene as mediator. Assay conditions were pH, 7; enzyme content, 10%; and applied voltage, 0.16V. Optimal working conditions were determined. The dispersion of enzyme in graphite was characterized using scanning electron microscopy (SEM, JEOL 6300F).The scanning electron microscopic (SEM) images were taken of the graphite surface before and after immobilization. Results indicate that ascorbic acid is a major interferent at the chosen potential. The reduction of ascorbic acid was performed enzymatically by ascorbate oxidase, previous to glucose determination. SEM were determined. The dispersion of enzyme in graphite was characterized using scanning electron microscopy (SEM, JEOL 6300F).The scanning electron microscopic (SEM) images were taken of the graphite surface before and after immobilization. Results indicate that ascorbic acid is a major interferent at the chosen potential. The reduction of ascorbic acid was performed enzymatically by ascorbate oxidase, previous to glucose determination. SEM voltage, 0.16V. Optimal working conditions were determined. The dispersion of enzyme in graphite was characterized using scanning electron microscopy (SEM, JEOL 6300F).The scanning electron microscopic (SEM) images were taken of the graphite surface before and after immobilization. Results indicate that ascorbic acid is a major interferent at the chosen potential. The reduction of ascorbic acid was performed enzymatically by ascorbate oxidase, previous to glucose determination. SEM 107 micrographs were employed to demonstrate the importance of surface properties in determining the response of the amperometric biosensor for glucose determination. Results also indicated that determination of glucose content in commercial apple juice with both methods (standard and biosensor) is statistically equivalent. In conclusion, amperometric biosensor shows good sensitivity and stability, and resulted strong enough to be applicable in real industrial conditions.