IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Interaction Between Purine Metabolism and Polyketide Production In Streptomyces coelicolor
Autor/es:
NAVONE L; CASATI P; GRAMAJO H; RODRIGUEZ E
Lugar:
San Miguel de Tucuman
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigadores en Bioquímica y Biología Molecular (SAIB); 2009
Resumen:
We have previously identified a cluster of genes sco6243, sco6247 and sco6248 from S. coelicolor encoding putative proteins involved in allantoin metabolism which are negatively regulated by Sco6246 (AllRStr), a protein homologue to the allantoin regulator protein AllR from E. coli. Genetic and transcriptional analysis studies have demonstrated that AllRStr encode a transcriptional repressor of this gene cluster. The inactivation of this protein strongly impairs antibiotic production. In order to check if increases of expression of the cognate genes of this pathway (for example sco6243, sco6247 or sco6248) are the reason for the impaired antibiotic production we are constructing mutant strain for each of these genes on an allRStr mutant background. In addition, we have made proteomic studies in order to identify other putative proteins that can be under control of AllRStr. In this way we have found several proteins, from purine metabolism and others involved or related to the acid tricarboxylic cycle, with an increase expression in the allRStr mutant strain compared to the S. coelicolor M145. This experiment also allowed verifying that Sco6243 were overexpressed in the allRStr mutant strain. These results could suggest that availability of precursors for synthesis of ppGpp or acetyl-CoA could be a check point for the production of secondary metabolites in Streptomyces.