IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Catalase gene family expresión in tomato fruits (cv. Micro-Tom) after postharvest chilling
Autor/es:
RÉ, M.; BOGGIO SB
Lugar:
Tucumán, Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Resumen:
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ROLE OF CATALASE IN
PROTECTING TOMATO FRUITS (CV. MICRO-TOM) FROM CHILLING INJURY.
Martín Ré y Silvana Boggio
IBR-CONICET, FCByF-UNR,
Suipacha 531, Rosario, Argentina. E-mail:
boggio@ibr.gov.ar
Cold storage of fruits can
alter the normal ripening process because of their susceptibility to chilling
injury. Oxidative stress has been associated with the appearance of this
physiological disorder and some antioxidants or free radical scavengers have
been shown to reduce it. Catalase (CAT) in plants has been associated with the
removal of H202 during stress conditions. We have
evidence suggesting that CAT could be responsible for chilling tolerance
observed in dwarf tomato cultivar Micro-Tom. The aim of this study was to
elucidate the role of different CAT genes during postharvest chilling stress in
Micro-Tom fruits. We found a new putative CAT gene by bioinformatics screening which
has high homology with other plant catalase genes and we named it cat3 from S. lycopersicum. Mature green fruits were harvested, stored at 4ºC during four weeks and transferred
to 25ºC. There
was an increase in CAT activity one day after the fruits were transferred to room
temperature but not during the cold storage. Also, we have analyzed the
expression of cat3 and three
different CAT genes previously identified (cat1,
cat2 and ER60). cat1 was the most abundant CAT gene expressed in mature green fruits
and we observed a great increase of cat1
transcripts one day after the fruits were transferred to 25ºC. Our results suggest that
cat1 plays a key role in chilling
stress tolerance, scavenging H2O2 after the cold storage
of Micro-Tom fruits.