Genome sequence analisys of Lactobacilli strain isolated from fermented animal food

ESPARIZ, M; BLANCATO, VS; MORTERA, P; MAGNI, C

San Miguel de Tucumán

Simposio; V Simposio Internacional de Bacterias Lácticas (SIBAL); 2016

CERELA-CONICET

Lactobacilli are a common lactic acid bacteria (LAB) widely studied in regard to their role in the dairy technology and probiotic properties. New isolated strains from specific environments, allows us to identify physiological capacities associated with novel metabolic networks. A Lactobacilli strain, named PM07, was isolated from fermented cereal food used for livestock feeding in the north of Santa Fe. One of our biotechnological aims, comprises to find fibrinolytic genes (xylanase, cellulase, chitinase or amylase) and another catabolic pathways present in this strain associated to vegetal fermentation.Illumina technology was used for sequencing; then SeqMan NGen software, Advanced Pipmaker and Mauve Genome Alignment for genome sequences assembly. Phylogenetic genome analysis, performed with Gegenees was not conclusive since the strain PM07 had 84-86% similarity with 11 Lb. casei and 84-85% with 2 Lb. paracasei strains. The similarity with the model strain Lb. casei BL23 was 84%. Genome size was 2,995,822 bases in length with 46.4% GC content, containing 2,975 coding sequences (CDS) and 71 RNAs (61 tRNAs). The Genome annotation, achieved by RAST assigned 1,276 protein encoding genes (43%) in 341 subsystems. As most LAB, Lb. PM07 strain shows a wide sugar fermentation capacity, represented by 425 CDS related to carbohydrate metabolisms. Specifically, a total of 10 genes encoding enzymes for maltose and maltodextrin utilization, and 26 genes related to lactose and galactose uptake and utilization, 12 of them are in a cluster organization. Chitin and N-acetylglucosamine utilization were assigned to the presence of 14 genes. Likewise, a total of 11 genes were associated to N-acetylgalactosamine and galactosamine metabolism. Instead, no genes related to cellulose metabolism were detected.Noteworthy, we found an 8 genes cluster encoding among others proteins, an aspartate aminotransferase family enzyme, a putative transport system permease protein, phosphopentomutase like enzyme, phosphotriesterase, and evolved beta-D-galactosidase. Most of these genes are only present in a few Lactobacilli strains (Lactobacillus casei LOCK919, Lb. paracasei ATCC 334 and 2 strains of Lb. rhamnosus). Moreover, we also detected 35 CDS corresponding to phage/prophage machinery. PHAST analysis tool revealed at least 4 prophage regions in the genome. Non plasmidic, virulence factors or human pathogenic sequences were found using PlasmidFinder, VirulenceFinder and PathogenFinder genomic analysis software. Additional bioinformatic and physiological works are needed to establish the phylogenic identity of Lactobacillus PM07 strain as well as to evaluate its technological potential for the production of fermented feed.