El impacto potencial del óxido nítrico sobre la asimilación de nitrógeno en plantas

NEJAMKIN ANDRÉS; MAYTA MARTÍN; LORENZO LAMATTINA; FORESI NOELIA ; DEL CASTELLO FIORELLA; CARRILLO NÉSTOR; CORREA-ARAGUNDE NATALIA; LODEYRO, ANABELLA

Corrientes

Congreso; XXXI Reunión Argentina de Fisiología Vegetal; 2016

Sociedad Argentina de Fisiología Vegetal

The potential impactof nitric oxide in nitrogen assimilation in plants El impacto potencial del óxido nítrico sobre laasimilación de nitrógeno en plantas  Foresi Noelia 1,Nejamkin Andrés1,  Correa-AragundeNatalia1, Del Castello Fiorella1, Mayta Martín2,Lodeyro Anabella 2, Carillo Néstor 2 y Lamattina Lorenzo11Instituto de InvestigacionesBiológicas, Universidad Nacional de Mar del Plata, CC1245, Mar del Plata,Argentina2Instituto de Biología Molecular yCelular de Rosario (IBR), CONICET-UNR. CP 2000, Rosario, ArgentinaResearch on Nitric Oxide (NO) in plants has gainedconsiderable attention in recent years mainly due to its function as a keysignaling molecule in different intracellular processes in plant growth anddevelopment. In higher plants, there are at least two enzymatic sources of NOproduction: (i) nitrate reductase, that reduces nitrate to nitrite, and thennitrite to NO, and (ii) a putative NO Synthase (NOS-like) enzymatic activity,although no NOS gene or protein was found yet. In animals NOS catalyzes theformation of NO and citrulline from L-arginine in a reaction that uses NADPH asan electron donor and O2 as a co-substrate. In the Lamattina?s labwas characterized the first NOS in the plant kingdom from the unicellular algaeOstreococcus tauri (OtNOS). OtNOS possessesmost of the characteristics ascribed to animal NOS. We transformed Nicotiana tabacum and Arabidopsis thaliana plants with OtNOS gene and studied the behaviour ofdifferent transgenic lines. Results indicate that a NOS-dependent NO productionimproves the growth, developmental and metabolic parameters of plants. Thesefindings are reminiscent of a concerted action of NO that also includes anincrease of N metabolism that might lead to improved nitrogen assimilation.