IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genetic relationship among North-West argentinean strains of Xac causing CBC.
Autor/es:
SICILIANO, FLORENCIA; PINO DELGADO, NATALIA; FAVARO, MARÍA ALEJANDRA; ENRIQUE, RAMÓN; SENDÍN, LORENA; VOJNOV, ADRIÁN; CASTAGNARO, ATILIO; MARANO, MARIA ROSA
Lugar:
Córdoba
Reunión:
Otro; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2008
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Forty-two strains of Xanthomonas axonopodis pv. citri (Xac), the causal agent of  bacterial canker of citrus, isolated from Citrus limon in north-western Argentina, were characterized phenotypic, biochemistry, and molecularly. The colonies of most isolates were mucoid, convex and yellow on nutrient agar plates as other xanthomonads, except for strain 44, which produced structures type biolfim. Simultaneously, with the confirmation of pathogenicity, the isolates were identified to species level by analysis of hydrolysis of gelatine and carbon source utilization. Molecular analysis were also performed using a previously described primer designed to be universal for all citrus canker strains and a new set of primers, that we developed, for specific identification of CBC type A strains. This primer is based on a sequence within xps gene of Xac str. 306, which encodes for the general secretion pathway protein D. All strains characterized were identified as Xac. Pathogenicity tests revealed that only str. 44 was unable to produce canker on lemon leaves. To evaluate the diversity of Xanthomonas strains causing CBC in NOA and to make a deeper characterization of str. 44 we performed DNA fingerprinting analysis using rep-PCR. This result was used to build a dendogram that shows the close relationships among Xanthomonas strains. Further analysis must be done to understand the less pathogenicity of str. 44.