IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CLINICAL RELEVANCE AND TISSUE TROPISM OF THE MUPAPILLOMAVIRUS GENUS TYPES HPV1, HPV63 AND HPV204
Autor/es:
BOTJAN J. KOCJAN; ELISA M. BOLATTI; STERBENC ANJA; ADRIANA A. GIRI; MARIO POLJAK; LEA HONJAK; DIEGO CHOUHY
Lugar:
Salzburg
Reunión:
Congreso; Eurogin 2016; 2016
Resumen:
Background: Mupapillomavirus (Mu-PV) genus currently consists of only 3 members: HPV1, HPV63 and HPV204. HPV1 and HPV63 were identified in 1980 and 1993, respectively, and are associated with the development of common warts. HPV204 is a novel Mu-PV type identified in 2014 with a yet undetermined clinical significance and tissue tropism (1). Objectives: To determine the clinical relevance and tissue tropism of HPV1, HPV63 and HPV204. Methods: Quantitative HPV1/HPV63/HPV204 type-specific real-time PCR assays with a sensitivity of at least 10 viral copies/assay were developed and used to test a representative collection of various HPV-associated benign and malignant neoplasms of the skin and mucosa, clinically normal mucosal samples and eyebrow hair follicles (n=1,006). HPV1, HPV63 and HPV204 viral loads per single human cell were estimated for all tissue samples. Results: HPV1 was detected in 2/110 (1.8%) of nasopharyngeal swab samples, 21/43 (48.8%) of common warts, 1/110 (0.9%) of eyebrow hair follicles and 3/40 (7.5%) of anogenital warts, HPV63 in 2/105 (1.9%) of oral mucosa swab samples, 3/110 (2.7%) of nasopharyngeal swab samples, 9/43 (20.9%) of common warts, 4/110 (3.6%) of eyebrow hair follicles and 1/110 (0.9%) of anal canal swabs, while HPV204 was detected only in 1/116 (0.9%) of cervical swab samples, 1/43 (2.3%) of common warts, 4/110 (3.6%) of anal canal swabs and 5/110 (4.5%) of penile surface swabs. HPV1 viral load ranged from 5.9 x 10-6 to 3.2 x 10-5 copies/cell in anogenital warts, 2.6 x 10-5 to 5.2 x 104 copies/cell in common warts and was 1.5 x 10-5 copies/cell in an eyebrow hair follicle. HPV63 viral loads ranged from 2.5 x 10-6 to 3.4 x 10-5 copies/cell in eyebrow hair follicles and from 3.2 x 10-6 to 3.9 x 102 copies/cell in common warts. Viral load of HPV204 in a common wart was low (7.3 x 10-7 copies/cell). Conclusions: Our study has expanded the current knowledge of tissue tropism of Mu-PV types. Although the prevalence of Mu-PV types is generally low (1.1%-2.7%), HPV1, HPV63 and HPV204 can be detected in virtually all types of benign cutaneous and mucosal samples, suggesting that all members of Mu-PV genus exhibit dual tissue tropism. On the contrary, none of the Mu-PV types were found in malignant neoplasms. HPV204 appears to cause only latent infections of the skin and mucosa, though further studies are needed to evaluate its potential role in the development of common warts.