CONICET | Buscador de Institutos y Recursos Humanos

The 2-Cys Peroxiredoxin B (2-Cys Prx B) is a chloroplastic protein that reduces a broad spectrum of peroxides like H2O2, lipid hydroperoxide and peroxinitrite. The 2-Cys Prx proteins are proposed to have chaperone activity as well. To understand the role of this protein in the defence system of plants we studied the activity of 2-Cys Prx B promoter by inserting the putative promoter region in the pBI101 plasmid and transforming Arabidopsis thaliana (Ecotype Col-7), setting the b-glucuronidase gene (GUS) under the control of the promoter. We analyzed the expression of the reporter gene under several stress conditions, such as heat, cold, osmotic stress, salinity stress, oxidative stress and fungal elicitors. Results indicated that 2-Cys-Prx B promoter region is highly active in leaves under control conditions. In addition, expression levels change under some stresses. These results suggest that 2-Cys Prx B could be involved not only in the antioxidant response but also in the response to other stresses in Arabidopsis thaliana.