USING METABOLOMIC APPROACHES TO EXPLORE DIVERSITY WITHIN HEALTHY POPULATION FROM THE CENTRAL REGION OF ARGENTINA.

BURDISSO PAULA; VAZQUEZ MARTIN; SCIARA MARIELA; RASIA, RODOLFO M.; COSCIA ANDREA; VILA ALEJANDRO

Rosario

Simposio; Latin American Metabolic Profiling Symposium; 2016

IBR-CONICET

Metabolomics is the large-scale study of small molecules, commonly known as metabolites, within cells, biofluids, tissues or organisms. The non-invasive nature of this technique allows the global study of metabolic profiles assuring the characterization of an individual metabolic phenotype. Metabolic phenotypes are the products of interactions among a variety of factors like genetics, diet, lifestyle, environment and gut microbiome. In order to investigate metabolic phenotype diversity across and within healthy people from the central region of Argentina, we conducted an NMR-based metabolomics analysis on biofluids samples collected from healthy volunteers with complete metadata records including 18 biochemical tests, lifestyle and diet factors and gut microbiome analysis. We analysed spectra from plasma and urine specimens for each of 150 participants from the project: ?Argentine Gastrointestinal Microbiome and Metabolome? involving 4 population samples aged 18-50 in Rosario, Paraná, Rafaela and Venado Tuerto. CPMG and NOESY spectra were pre-processed and spectral data was extracted for multivariate data analysis. PCA and OPLS-DA models were constructed. As we expected, urinary and blood metabolite patterns for each region samples are not significantly differentiated, nevertheless we could detect patterns between outliers and the associated metadata. The present work defines criteria and variability within the local population that will have to be considered in future studies.