IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Mice immunization with live lactococci expressing rotavirus VP8 antigen
Autor/es:
MARELLI, BELKIS; DE MENDOZA, DIEGO; MAGNI, CHRISTIAN
Lugar:
Villa Carlos Paz- Córdoba
Reunión:
Congreso; XLIV Reunión Anual de SAIB; 2008
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
In the last decade, several studies have used lactic acid bacteria as antigen delivery vehicles to develop safe, live vaccine. Recombinant Lactococcus lactis strains expressing heterelogous antigens have been successfully used to elicit an immune response against bacterial or viral antigens. In this context, we use L. lactis for the expression of rotavirus antigens in order to develop an useful tool for immunization against this virus. For this purpose, we constructed two recombinant L. lactis strains targeting VP8 antigen to the cytoplasm (LL-VP8cyt) or to the cell wall (LL-VP8cwa). The expression capacity of such strains was analyzed by Western blot. Then, their ability to induce an immune response in mice was compared. Groups of eight Balb/c female mice were immunized intragastrically with the induced L. lactis strains. Control mice received identical quantities of wild type L. lactis (LL). After the last boosting, mice were sacrificed and levels of specific serum IgG and intestinal IgA against VP8 were measured by ELISA and Western blot. In this way, it was determined that the group of immunized mice with LL-VP8cyt produced a level of IgA significantly higher than the control. On the other hand, the group that received the LL-VP8cwa strain showed both increased levels of intestinal IgA and serum IgG. These strains are thus good candidates for the immunization against rotavirus.