GLOBAL GENOMIC DIVERSITY OF HPV6 AND HPV11

JELEN MM; CHEN Z; KOCJAN BJ; BURT FJ; CHAN PKS; CHOUHY D; COMBRINCK CE; COUTLÉE F; ESTRADE C; FERENCZY A; FIANDER A; FRANCO EL; GARLAND SM; GIRI AA; GONZÁLEZ JV; GRÖNING A ; HEIDRICH K; HIBBITTS S; HONJAK L; LUK TNM; MARINIC K; MATSUKURA T; NEUMANN A; OTRBENK A; PICCONI MA; RICHARDSON H; SAGADIN M; SAHLI R; SEEDAT RY; SEME K; SEVERINI A; SINCHI JL; SMAHELOVA J , ; TABRIZI SN; TACHEZY R; TOHME S; ULOZA V; VITKAUSKIENE A; WONG YW; IDOVEC LEPEJ S; BURK RD; POLJAK M

Sevilla

Congreso; EUROGIN 2015; 2015

EUROGIN

OBJECTIVES To investigate the global HPV6/11 genomic diversity and phylogenetic relationships among the most variable HPV6/11 complete genome (CG) variants. METHODS In total, 530 HPV6 and 207 HPV11 isolates from the anogenital and head and neck anatomical regions were obtained from six continents. Isolates were analysed in regions: E5a-E5b-L1-LCR and the most divergent were selected for CG sequencing. We constructed maximum likelihood (RAxML) phylogenetic trees, assigned (sub)lineages, identified (sub)lineagespecific SNPs and statistically evaluated the geographical and clinical associations among HPV6/11 genomic variants. RESULTS In total, 130 and 14 CGs were obtained for HPV6 and HPV11, respectively. Pairwise heterogeneities of 190 HPV6 CGs (130 from our study, 60 from GenBank) and 63 HPV11 CGs (14 from our study, 49 from GenBank) were 1.6% and 1.4%, respectively. Global phylogenetic trees revealed two lineages (A and B) and five sublineages: B1-B5 for HPV6 and two lineages A and B and four sublineages A1-A4 for HPV11. Among HPV6 variants lineage B prevailed globally, lineage A in Asia and sublineage B3 in Africa and Americas. Sublineages B1 and B3 were associated with anogenital infections and B3 showed higher odds for infection in females. Among HPV11 variants sublineage A2 prevailed globally, while newly assigned lineage/sublineages B, A3 and A4 consisted of one African, one North American and one European variant, respectively. CONCLUSIONS This is the first extensive work on globally circulating HPV6/11 genomic variants. Preliminary results suggest the existence of novel HPV6/11 lineage/sublineages, with HPV6 variants exhibiting some degree of ethnogeographic, gender and/or disease predilection in their distribution.