IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
GENOMIC CHARACTERIZATION AND TISSUE TROPISM OF A POTENTIALLY NOVEL MUPAPILLOMAVIRUS ISOLATED FROM THE ANAL CANAL
Autor/es:
B. J. KOCJAN; L. HOSNJAK; D. CHOUHY; A. A. GIRI; M. POLJAK
Lugar:
Nueva Orleans
Reunión:
Congreso; 115th General Meeting of the American Society for Microbiology; 2015
Institución organizadora:
American Society for Microbiology
Resumen:
Background: Mupapillomavirus (Mu-PV) genus currently contains of only two HPV types: HPV1 andHPV63 identified in 1980 and 1993, respectively, both exhibiting skin tissuetropism and association with sporadic cases of plantar warts. Objectives: Inthis report we describe isolation, genomic characterization and tissue tropism of anovel Mu-PV HPV204. Methods: PartialHPV204 L1-gene sequence similar to both Mu-PVs wasinitially identified in 2009 in a skin swab sample using CUT primers. A completeviral genome was amplified in 2014 from an anal swab sample of a 36-yearold male patient with HPV53-positive anal warts usinginverse long-range PCR, cloned, and sequenced by the primer walking strategy. HPV204type-specific quantitative real-time PCR was developed and used to determinethe prevalence of a novel type on a collection of skin, mucosal andmucocutaneous clinical samples. Results: Genome of a novel Mu-PV comprises 7,227-bp, andcontains five early (E1, E2, E4, E6 and E7) and two late genes (L1 and L2), andthe LCR genomic region positioned between L1 and E6 ORFs. With an L1 nucleotide sequence identityof 66.3% and 66.7% to HPV1 and HPV63, respectively, HPV204 is classified as anew type in a new Mu-PV speciesMu-3. Novel type was further detected in 6/115 skin warts, 4/110 analcanal samples, and in none of the 120 oral cavity samples. Conclusion: Full characterization of HPV204 expands the current knowledge of geneticdiversity and tissue tropism of Mu-PV genus. The clinical significance of HPV204 infections is currently under investigation.