IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differences in the age-related immuno-endocrine response in rats after T. cruzi challenge
Autor/es:
MANARIN ROMINA; PÉREZ ANA ROSA; BOTTASSO OSCAR; REVELLI SILVIA
Lugar:
Buenos Aires, Argentina
Reunión:
Congreso; III Iberoamerican Congress on Neuroimmunomodulation; 2009
Resumen:
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We previously demonstrated that T. cruzi-infected weaning rats (21-day
old) were more susceptible to infection than their adult counterparts (aged 70-days). To gain a further insight on this
age-related susceptibility, we evaluated some features of the inflammatory
response elicited either by T. cruzi infection
or LPS (mimetic to T. cruzi-GPI
anchors) challenge in weaning and adult rats.
LPS inoculation in weaning rats led
to a 50% mortality and an increased IL-10 production respect adult-challenged
rats (overall p<0.02). Opposite cytokine profiles were seen during T. cruzi infection, with TNF-a levels being higher in weaning rats
(90 h p.i.; p=0.016), and IL-10 showing increased values in adult animals (24 h
p.i.; p=0.029). Accompanying these findings, corticosterone and TGF-b levels detectable in both infected
groups were more elevated in weaning animals (p<0.05). Noticeably, younger rats had
a decreased ability to control parasitemia compared with adult animals. In
vitro studies evidenced that weaning rat-derived peritoneal macrophages exposed
to LPS produced higher TNF-a levels in comparison to whose derived from adult counterparts (p=0.05).
The greater susceptibility of weaning rats to the T. cruzi infection seems to be related to a different profile of the
inflammatory reaction, also involving the adrenal endocrine response.