IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
XacPNP from Xanthomonas citri sp. citri resemble the function of Arabidopsis thaliana PNP in plants
Autor/es:
GRANDELLIS C; FICARRA F; GOTTIG N; OTTADO J
Lugar:
Foz do Iguacu
Reunión:
Congreso; IPMB 2015; 2015
Resumen:
Xanthomonas citri subsp citri is the pathogen that provokes citrus canker affecting the yield hence causing economic loses in cultivation. The study of this plant-pathogen interaction can lead to develop solutions to control the disease. Plant natriuretic peptides (PNPs) are systemically mobile molecules have been reported in Citrus sinensis and Arabidopsis thaliana, as well as many plant species, and they have a role in the maintenance of plant homeostasis. Xanthomonas citri subsp citri also contains a gene encoding a PNP-like protein (Gottig 2008), named XacPNP. XacPNP is an active protein from the pathogen that upon infection elicits a plant defense response, and is expressed in bacteria during citrus plant-pathogen interaction (Gottig, 2008). The expression of defense genes in Arabidopsis thaliana plants previously infiltrated with recombinant XacPNP was analyzed, and the results showed defense genes were up-regulated. Xanthomonas enters the host through stomata thus infecting the tissue and we previously reported that treatment with XacPNP resulted in rapid and significant increase in stomatal conductance (Gottig et al., 2008). To analyze the function of XacPNP in the plants, transgenic Arabidopsis thaliana plants were constructed overexpressing the bacterial protein XacPNP. Transgenic lines were obtained by floral dip Agrobacterium tumefaciens mediated plant transformation, and fully evaluated at both molecular and phenotypic levels. When evaluating the transgenic lines, we found that stomata were open in XacPNP overexpressing plants when compared to wild types plants, in accordance to previous results. This demonstrates that XacPNP is able to emulate AtPNP, and confers the phytopathogen an advantage during the infection.