IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MOLECULAR CHARACTERISATION AND TISSUE TROPISM OF A NOVEL MUPAPILLOMAVIRUS HPV204
Autor/es:
BOTJAN J. KOCJAN; LEA HONJAK; ANJA TERBENC; DIEGO CHOUHY; ELISA BOLATTI; ADRIANA A. GIRI; MARIO POLJAK
Lugar:
Lisboa
Reunión:
Conferencia; 30th International Papillomavirus Conference; 2015
Resumen:
Background: Mupapillomavirus (Mu-PV) genus currently contains of only two HPV types: HPV1 and HPV63 identified in 1980 and 1993, respectively, both exhibiting skin tissue tropism and association with sporadic cases of plantar warts. Objectives: In this report we describe isolation, genomic characterization and tissue tropism of a novel Mu-PV HPV204. Methods: Partial HPV204 L1-gene sequence similar to both Mu-PVs was initially identified in 2009 in a skin swab sample using CUT primers. A complete viral genome was amplified in 2014 from an anal swab sample of a 36-year old male patient with HPV53-positive anal warts using inverse long-range PCR, cloned, and sequenced by the primer walking strategy. HPV204 type-specific quantitative real-time PCR was developed and used to determine the prevalence of a novel type on a collection of skin, mucosal and mucocutaneous clinical samples. Results: Genome of a novel Mu-PV comprises 7,227-bp, and contains five early (E1, E2, E4, E6 and E7) and two late genes (L1 and L2), and the LCR genomic region positioned between L1 and E6 ORFs. With an L1 nucleotide sequence identity of 66.3% and 66.7% to HPV1 and HPV63, respectively, HPV204 is classified as a new type in a new Mu-PV species Mu-3. Novel type was further detected in 6/115 skin warts, 4/110 anal canal samples, and in none of the 120 oral cavity samples. Conclusion: Full characterization of HPV204 expands the current knowledge of genetic diversity and tissue tropism of Mu-PV genus. The clinical significance of HPV204 infections is currently under investigation.