IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of Salmonella effectors on the regulation of eukaryotic molecular motors and vesicular trafficking.
Autor/es:
DIACOVICH L, SCHROEDER N, DUMONT A, LAFITTE D, SOPRANO E, BIGNON C, GORVEL JP, BOURNE Y, MÉRESSE S.
Lugar:
Centre d'Immunologie de Marseille-Luminy (CIML). Francia.
Reunión:
Workshop; EMBO Workshop: Visualizing Immune System Complexity. A multidisciplinary approach for a quantitative description of molecular and cell dynamics.; 2009
Institución organizadora:
European Molecular Biology Organization (EMBO).
Resumen:
Salmonellosis is one of the most common and widely distributed foodborne diseases. In last years problems related to Salmonella have increased significantly, both in terms of incidence and severity of cases of human salmonellosis. Also, the emergence of multidrug-resistance Salmonella strains with resistance to fluoroquinolones and third-generation cephalosporins is a serious development, which results in severe limitation of the possibilities for effective treatment of human infections. The virulence of this pathogen relies on its ability to establish a replicative niche, named the Salmonella-containing vacuole (SCV), inside host cells. The understanding of the molecular interactions between Salmonella effectors and host target will provide evidence of how effectors activate a cellular signalization pathway and define its role during the course of the pathogenic process. Here we propose the study of the action of three particular TTSS-2 effectors: SifA, PipB2 and SopD2. We characterized, at biochemical and structural level, the interaction between SifA and the eukaryotic protein SKIP that regulates the kinesin activity on SCV. In addition we characterized the interaction between PipB2 and kinesin. Furthermore, we analized the function of SopD2 in the regulation of SCV membrane dynamics and the intracellular replication of the bacteria.