IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CNBP is phosphorylated by PKA during zebrafish embryonic development
Autor/es:
LOMBARDO, V. A.; ARMAS, P.; CALCATERRA, N.B.
Lugar:
Cancún, México.
Reunión:
Congreso; First Pan American Congress in Developmental Biology. 66th Annual Meeting of the Society for Developmental Biology (SDB); 8th Annual Meeting of the Mexican Society for Developmental Biology (SMBD); 3rd International meeting of the Latin American Society.; 2007
Institución organizadora:
LASDB; SDB; SMBD
Resumen:
Cellular Nucleic acid Binding Protein (CNBP) was reported as a protein essential for normal forebrain development in mouse (Chen et al., 2003) and chicken (Abe et al., 2006). Zebrafish cnbp is expressed in the anterior CNS in a similar way to the observed in early chicken and mouse embryos. CNBP knockdown cause neural crest derivative depletion in zebrafish embryos. Collagen 2a1 (col2a1) in situ hybridization on these embryos showed defects in the formation of facial cartilage (Weiner et al., submitted). On the other hand, Sox9 function, a transcriptional factor involved in early events of neural crest development, is promoted by cAMP-dependent Protein Kinase (PKA) activity. This modification enhances Sox9 function in the transcriptional activation of col2a1 enhancer/promoter in cartilage cell (Huang et al. 2000). To analyse if CNBP is post-translationally regulated by phosphorylation during the embryogenesis of vertebrate, we carried out in vitro phosphorylation assays using zebrafish embryonic extracts and observed that CNBP was differentially phosphorylated during embryogenesis. PKA was identified as the predominant kinase that phosphorylates on the unique CNBP putative conserved phosphorylation site. Then, this post-translational modification may not only exist in zebrafish but also in other vertebrates. Finally, we observed that CNBP phosphorylation modified its biochemical activities (Lombardo et al., 2007). It seems likely that phosphorylation might be a conserved post-translational modification that allows CNBP to perform a fine tune of the expression of a group of genes during neural crest development.