IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Analysis of a 2-cys peroxiredoxin b overexpressing Arabidopsis thaliana line
Autor/es:
BONDINO, HG; SCARPECI, TE; VALLE, EM
Lugar:
Mar del Plata
Reunión:
Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2007
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
The 2-Cys peroxiredoxin B (2-Cys Prx B) is a chloroplastic protein that reduces a broad spectrum of peroxides like H2O2, lipid hydroperoxide and peroxinitrite. Its peroxidase activity was extensively analyzed in vitro in the literature, but studies are lacking about its in vivo function.To understand the role of this protein in the antioxidant defence of plants we overexpressed the 2-Cys Prx B gene (At5g06290) under the control of the cauliflower mosaic virus 35S promoter (CaMV 35S promoter) in Arabidopsis thaliana (Ecotype Col-7).We analyzed the constitutive transcript levels of several defence related genes in an overexpressing line (OE-line). Apparently, overexpression of 2-Cys Prx B in Arabidopsis thaliana did not significantly affect the mRNA levels of genes of the antioxidant system.We also studied the participation of 2-Cys Prx B in the tolerance to oxidative stress in Arabidopsis thaliana by treatment with methyl viologen (MV). We determined electrolyte leakage, chlorophyll content and levels of antioxidant enzymes. Results indicated higher tolerance of the OE-line to this oxidative challenge.These results suggest that 2-Cys Prx B could be involved not only in keeping redox homeostasis of chloroplasts but also in the antioxidant response of Arabidopsis thaliana.