IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
GROUP Pseudomonas putida: A SILENT ENVIRONMENTAL RESERVOIR OF ANTIMICROBIAL RESISTANCE GENES
Autor/es:
BROVEDAN M; MARCHIARO, P.; DIAZ S.; PASTERAN FERNANDO,; MORAN BARRIO, J.; VIALE A.M.; LIMANSKY ADRIANA S.
Lugar:
Rosario
Reunión:
Congreso; 50th Annual Meeting SAIB; 2014
Institución organizadora:
SAIB
Resumen:
The Pseudomonas putida group encompasses at least 12 environmental species, from which P. putida and P. monteilli represent opportunist pathogens exhibiting antimicrobial resistance. We conducted a taxonomic classification of carbapenem resistant clinical isolates of the P. putida group recovered from Argentina, and characterize genetic platforms carrying blaVIM-2. A phylogenetic tree employing housekeeping genes identified 7 species, including P. putida, P. monteilii, and 5 unreported species from 13 P. putida group isolates. Three different genetic platforms containing blaVIM-2 were observed as judged by PCR mapping and sequencing, all including an unusual class 1 integrons. InT7633 (1) was identified on 10 strains including P. putida, P. monteillii and three undescribed species. Moreover, pLD209-like conjugative plasmids (1) carrying InT7633 were found in 5 strains and mobilized to P. aeruginosa and Escherichia coli, suggesting that horizontal transfer (HTG) of blaVIM-2 was responsible of resistance dissemination. Overall results suggest that blaVIM-2 is mobilized by transposition, and disseminated by HTG among different members of the group. This work uncovers the ability of species of the P. putida group to represent silent environmental reservoirs of resistance genes and, to provide them to opportunist pathogens in the clinical settings. 1. Marchiaro et al. 2014. AAC 58:1816.