IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cell death in Citrus limon triggered by a new variant of Xanthomonas citri subsp. citri.
Autor/es:
ROESCHLIN, R.A.; FAVARO, M.A; CHIESA, M.A.; D´ANDREA, R.; VOJNOV, A.; ALEMANO, S.; FILIPPONE, P.; GMITTER JR., F; CASTAGNARO, A.; GADEA, J.; MARANO, M.R
Lugar:
Rhodes
Reunión:
Congreso; XVI International Congress on Molecular Plant-Microbe Interactions; 2014
Institución organizadora:
Molecular Plant Microbe Interaction (MPMI)
Resumen:
Citrus is an economically important fruit crop that is severely afflicted by Citrus Bacterial Canker, a disease caused by Xanthomonas citri subsp. citri (XC). Recently, we identified a variant of XC isolated from Argentina, named XC AT, which induces a hypersensitive-like response (HR) in Citrus limon leaves and weak cankerous lesions in C. clementina. In C. limon, suppression of canker development is concomitant with an oxidative burst and callose deposition, suggesting that bacterial factors would be involved in triggering the HR. Moreover, transcriptomic analysis of inoculated C. limon leaves revealed that XC AT triggers markers reminiscent of a HR, including specific genes that points towards the involvement of salicylic acid (SA) pathway. Liquid chromatography - mass spectrometry analysis confirmed the participation of SA in this plant-bacteria interaction. In addition, transmission electron microscopy analysis on C. limon leaves inoculated with XC AT, showed ultrastructural changes, such as vacuolization, elongation of chloroplasts and the appearance of double-membrane vesicles, that confirm the elicitation of a pathogen-associated cell death response. Elucidate the mechanisms which drive the host resistance to XC AT will allow the development of new strategies to manage the disease.