IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Divergence of triacylglycerols accumulation and antibiotics production in Streptomyces coelicolor
Autor/es:
ARABOLAZA, A; RODRIGUEZ, EDUARDO; ALTABE, SILVIA GRACIELA; GRAMAJO, H
Lugar:
Rosario
Reunión:
Congreso; XLII Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular (SAIB); 2006
Institución organizadora:
Sociedad Argentina de Bioquímica y Biología Molecular
Resumen:
The occurrence of triacylglycerols (TAGs) as reserve compounds is widespread among eukaryotic organisms. However, accumulation of TAGs in prokaryotes has been reported for bacteria mainly belonging to the actinomycetes group. In Streptomyces, accumulation of TAGs was propose to be necessary to maintain cell integrity after glucose becomes exhausted, and also to provide the carbon units needed for subsequent biosynthesis of polyketide antibiotics. Here we describe the identification and characterization of proteins from S. coelicolor involved in the biosynthesis of TAGs and its role on antibiotics production. Sequence comparison allowed identify three genes in the chromosome of S. coelicolor encoding putative acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymes which catalyze the final step in TAG biosynthesis. In vitro experiment using recombinant proteins allowed us to confirm that Sco0958 is a DGAT. Furthermore mutant strains for Sco0958 and Sco0123 genes were generated by genetic manipulation. Characterization of these mutant strains in rich liquid medium showed that they accumulated less amounts of TAGs and produced more polyketide antibiotics than the parent strain. These results suggest that TAG biosynthesis in Streptomyces is demanding acetate-derived unit rather than providing carbon units for antibiotics production as previously proposed