IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A novel NTRC transcription factor regulates chemotaxis, biofilm formation and virulence in Xanthomonas citri sbsp. citri.
Autor/es:
YARYURA P.M.; CONFORTE V.P.; MALAMUD F.; ROESCHLIN R.; DE PINO V.; CASTAGNARO A.P.; DOW M.; MARANO M.R.; VOJNOV A.A.
Lugar:
Mar del Plata
Reunión:
Congreso; VIII Encuentro Latinoamericano y del Caribe de Biotecnología - REDBIO ARGENTINA 2013.; 2013
Institución organizadora:
Rebio Argentina Asociación Civil
Resumen:
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is one of the mayor diseases producing great economic losses worldwide. Xcc ability to form biofilm structures on leaves is closely related to its virulence. Through a screening of mutants generated by the Tn5 transposon insertion in order to find those defective in biofilm formation, we found the ntrC mutant. This gene encodes a transcription factor that had not been described previously in Xcc. Here we demonstrated that the ntrC mutant is unable to form the wild type mature biofim structures. Its capacity to move toward grapefruit leaves extracts is severely reduced due to the absence of flagella as a consequence of the down-regulation of flagellar genes. Moreover, this ntrC mutant is less virulent than the wild type strain. Introduction of gene encoding an enzyme that can degrade cyclic 3',5'-diguanylate (c-di-GMP) into the ntrC mutant restored chemotaxis, motility and virulence to wild type levels. This remark suggests a possible relationship between NtrC and c-di-GMP in bacteria.