Transcriptome of grapefruit during citrus canker development using RNA-Seq

DAURELIO, L.D.; KRAISELBURD, I.; GONZALEZ, M.; TALÓN, M.; TADEO, F.; ORELLANO, E.G.

Ciudad Autónoma de Buenos Aires

Simposio; 32 New Phytologist Symposium. Plant interactions with other organisms: molecules, ecology and evolution; 2013

New Phytologist Trust

Citrus trees, one of the most important fruit crops in the world, are currently exposed to diverse pathogens. Xanthomonas citri subsp. citri (Xcc) produces citrus canker, one of the most damaging citrus disease worldwide. This disease affects all citrus species, producing losses in productivity Here we analyzed the citrus transcriptome in fruit peel tissue using RNA-Seq at 1, 3 and 5 days post treatment (dpt).The analysis to detect differential expressed genes (DEG) was performed with RobiNA software (http://mapman.gabipd.org/web/guest/robin) and the functional analysis with MapMan software (http://mapman.gabipd.org/web/guest/mapman).RNA-Seq analysis uncovered 787 DEG in at least one Xcc treatment in comparison to Ctr or between different times post treatments. No DEG were found between Xcc 1 dpt and Ctr while a high number of DEG were observed between Xcc 5 dpt and Ctr, in correlation with disease symptoms progress on the tissue. The functional categories of DEG included "regulation of expression" and "hormone metabolism" among others. This study presents a novel transcriptome RNA-Seq analysis during citrus canker disease, using fruits from trees grown under field conditions similar to those used for citriculture and revealed genes involved in citrus canker development that could be used in future strategies of molecular breeding for citrus disease resistance.