IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Localization of new components of the egg extracellular matrix of amphibians and fish
Autor/es:
OBRIEN, E.; KRAPF, D.; ARRANZ, SILVIA EDA; CABADA, M.
Lugar:
Rosario
Reunión:
Congreso; XLII Reunión anual SAIB; 2006
Resumen:
The importance of the oocyte extracellular matrix during fertilization has been studied in severalanimal species. However, many of its roles have not been elucidated, being sometimescontroversial. Four major glycoproteins have been reported in the Vitelline Envelope (VE) of Bufoarenarum egg: gp120, gp75, gp41 and gp38. Recently, we identified two proteins of 115 kDa(p115) and 106 kDa (p106), that are also present in the VE but possess different biochemicalcharacteristics. The present work is focused on the characterization and localization of these newcomponents and analyzes the existence of homologous counterparts in other animals. Antisera wereraised against each protein. P106 and p115 were detected both in oocyte plasma membrane and VEextracts. Same results were obtained by inmunohistochemistry (IHQ) studies of B. arenarum ovary.The plasma membrane inmunoreactivity was lost after fertilization, but fertilization envelope (FE)remained positively marked. IHQ studies of X. laevis and Danio rerio ovaries indicated positiveinmunoreactivity in both cases with same localization that in Bufo arenarum. The roles of p115 andp106 have not been elucidated yet, although it seems that both components are not exclusive of B.arenarum specie. Our preliminary results suggest that they could be involved in the VE adhesion toplasma membrane which is lost after fertilization.