IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Comparative and functional genomics applied to the study of PVX pathogenesis in Solanum tuberosum.
Autor/es:
QÜESTA J.; SICILIANO F.; SÁNCHEZ G.; ENRIQUE R.; MARANO M.R.
Lugar:
Rosario
Reunión:
Congreso; XLII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB).; 2006
Institución organizadora:
SAIB
Resumen:
Several resistance genes, including R1 against Phytophthora infestans and Nb against PVX-ROTH1, were previously mapped to a resistance gene cluster on the short arm of potato chromosome V. To explore the functional and evolutionary significance of clustering in the generation of novel disease-resistance genes, a physical map of the R1-Nb region was constructed for each of the three genomes of Solanum demissum. Sequence analysis of the region, predicts the presence of more than 40 resistance genes candidates (RGCs) belonging to distinct resistance-gene families: R1, Prf and Bs4. The S. demissum Bs4 homologue, is a highly structurally conserved Type II gene, and its localization is coincident with the position of Nb. Thus, we hypothesized that the Bs4 homologue present in S. tuberosum cv Pentland Ivory is a candidate Nb gene. To test these, we mapped the RGCs-Bs4 in a recombinant population of Nb. Genetic and molecular analysis show that one of the sequences belongs to Nb. We developed an agroinfiltration system to asses the transient expression of the viral elicitor, the 25 kDa protein. We analyzed the biochemical and molecular changes triggered by defense response such as the level of reactive oxygen intermediates and the expression of PR-1a in resistant and susceptible plants. Our results suggest that this system can be used for the functional complementation analysis of Nb-RGC.