IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
First steps in Aquaculture genetics in Argentina: development of microsatellites markers for three species using 454 pyrosequencing-based technology.
Autor/es:
VILLANOVA GABRIELA VANINA; VERA MANUEL; DIAZ JUAN; CALCATERRA NORA BEATRIZ; MARTINEZ PAULINO; ARRANZ SILVIA EDA
Lugar:
Auburn
Reunión:
Congreso; The International Symposium on Genetics in Aquaculture XI at Auburn University; 2012
Institución organizadora:
Auburn University
Resumen:
First steps in Aquaculture genetics in Argentina: development of microsatellites markers for three species using 454 pyrosequencing-based technology. Villanova G. V.1, Vera M.2, Diaz J.1, Calcaterra N. B.1, Martínez P.2 and Arranz S. E.1. 1IBR-CONICET, School of Biochemistry and Pharmaceutical Sciences- National University of Rosario, Suipacha 590, 2000 - Rosario, Argentina. TFN +543414356369. 2Genetics Department, School of Veterinary, Santiago de Compostela University, 27002 - Lugo, Spain. TFN/FAX +34982822428. villanova@ibr.gov.ar. Aquaculture production is playing an increasing role in supplying fish for human consumption. Although aquaculture is poorly developed in Argentina, it is experiencing a constant growth during the last twenty years. Argentina shares with Uruguay, Brazil and Paraguay the second biggest hydrographic region in South America, La Plata basin. Since its high biodiversity, La Plata basin houses essential resources, hence, the necessity of genetic studies for its conservation. In this study, microsatellites markers were identified and characterized for pacú (Piaractus mesopotamicus), boga (Leporinus obtusidens) and surubí (Pseudoplatystoma corruscans). Pacú production is the most important at the northeast Argentinean region, followed by surubí. Pacú fishing is forbidden in order to protect the species because of population decline. Surubí is the biggest fish in La Plata basin, and its meat is highly valuable. Boga culture started a few years ago. It has a promising future due to its acceptance in the domestic market. Using only 1/16 of a shotgun 454 pyrosequencing reaction for each species, we were able to obtain 2477, 2485 and 5579 microsatellite-containing sequences for boga, pacú and surubí respectively. Primers were designed for 262 pre-selected microsatellites in boga, 390 in pacú and 214 in surubí. Selected microsatellites showed a minimum number of repetitions and enough flanking regions for primer design. For each species, 40 loci were finally selected for further optimization. In boga, 20 loci were successfully genotyped in 19 wild specimens from Paraná River and showed between 2?20 alleles (A) per locus (mean = 5.75) and a mean expected heterozygosity (HE) of 0.568. In pacú, 18 loci were successfully genotyped in 50 farmed specimens, 14 microsatellite loci being polymorphic (2?5 A/locus; mean = 2.83; HE = 0.293). This low genetic diversity figures per locus may reflect the farming origin of samples and suggests improper broodstock management at farm facilities. In a similar way, 17 loci were successfully genotyped in 38 farmed surubí specimens, and only 14 microsatellite loci were polymorphic (2?10 A/locus; mean = 3.12; HE= 0.296). Currently, these 35 loci are being tested in wild specimens from Paraná River to compare genetic diversity figures with farmed populations. The molecular markers characterized in this work will be a valuable tool for stocks evaluation of farmed fishes, genetic variability measures and population structure studies of these Neotropical species.