IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Plant small heat shock proteins during different stress conditions other than heat. Comparative analysis between Arabidopsis thaliana and Solanum lycopersicum
Autor/es:
ARCE, DEBORAH; MARTÍN RÉ; SILVANA BOGGIO; ESTELA VALLE
Reunión:
Congreso; 3er Congreso Argentino de Bioinformática y Biología Computacional; 2012
Resumen:
Plant small heat shock proteins during different stress conditions other than heat. Comparativeanalysis between Arabidopsis thaliana and Solanum lycopersicumDébora Pamela Arce1, Martin Damián Ré2, Silvana Beatriz Boggio2 and Estela Marta Valle21 Facultad Regional San Nicolás, Universidad Tecnológica Nacional, San Nicolás, 2900, Buenos Aires,Argentina2 Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Universidad Nacional de Rosario,S2002LRK, Rosario ArgentinaBackgroundSmall heat shock proteins (sHSPs) are chaperones that play an important role in abiotic and biotic stresstolerance. The special importance of sHSPs in plants is suggested by their unusual abundance and theyare found in different cellular compartments1. Furthermore, some sHSPs are also expressed during certainstages of development2. In this study Arabidopsis thaliana and Solanum lycopersicum were used as modelplants. Previous findings allowed us to identify heat shock proteins (HSPs), heat shock factors (Hsfs) andsHSPs genes up-regulated under oxidative stress mediated by methyl viologen (MV) in Arabidopsis3. Inaddition, tomato sHSPs were induced in red fruit compared to green fruit4. All these results allowed us toperform an in silico strategy for analyzing the regulation of sHSPs gene expression. Three sHSPspromoter sequences from mitochondrial LeHsp23.8-M, cytosolic LeHsp17.7-CI and cytosolic LeHsp17.4-CII were analyzed.Materials and MethodsIn the present work we made a screening of the following databases: Sol genomics networkhttp://www.sgn.cornell.edu/], Tomato EST Database http://ted.bti.cornell.edu/], NCBI Databasehttp://www.ncbi.nlm.nih.gov/]. The Arabidopsis Information Resource (TAIR) [http://arabidopsis.org/].Sequence analyses were performed using bioinformatics tools BLASTn and T-Coffee5. For promoteranalysis, 1.9 kb upstream regions of sHsp genes were identified by BLASTN in NCBI database and in Solgenomics network (SGN Combined WGS-BAC-unigenes set). Heat shock elements (HSEs) weresearched in the PLACE database [http://www.dna.affrc.go.jp/PLACE/index.html]. Further analysis toidentify conserved motifs was performed using expectation maximization method MEME[http://meme.sdsc.edu/meme4/intro.html], PlantCARE database[http://bioinformatics.psb.ugent.be/webtools/plantcare/html/] and MatInspector program6.ResultsThe analysis of 1.9 kb promoter region of sHsp genes using bioinformatics tools (see Materials andMethods) showed that heat shock elements or HSEs (CCAAT Box) were present in the three sequencesanalyzed. Other motifs also detected were a related sequence of abscisic acid response element (ABRE)and ethylene responsive elements (ERE).ConclusionsThese results indicate that LeHsp23.8-M, LeHsp17.7-CI and LeHsp17.4-CII could be involved in differentprocesses mediated by some plant hormones (abscisic acid or ethylene) other than heat stress. Thesepromoter sequences could be used in the generation of tomato transgenic plants to evaluate theLeHsp23.8-M, LeHsp17.7-CI and LeHsp17.4-CII gene expression pattern under environmental stressconditions or different developmental stages.References1. Wang W, Vinocur B, Shoseyov O, Altman A: Role of plant heat-shock proteins and molecularchaperones in the abiotic stress response. Trends Plant Sci 2004, 9:244-252.2. Prasinos C, Kampis K, Samakovli D, Hatzopoulos P: Tight regulation of expression of twoArabidopsis cytosolic Hsp90 genes during embryo development. J Exp Bot 2005, 56:633-644.3. Scarpeci TE, Zanor MI, Carrillo N, Mueller-Roeber B, Valle EM: Generation of superoxide anion inchloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly inducedgenes. Plant Mol Biol 2008, 66(4):361-3784. Re MD, Arce DP, Boggio SB: Expresión de sHsps luego de la conservación en frío de tomates (cv.MICRO-TOM). V Jornadas argentinas de Biología y Tecnología Postcosecha, 2009.5. Notredame C, Higgiens D, Heringa J: T-Coffee: A novel method for multiple sequence alignments.J Mol Biol 2000, 302: 205-217.6. Cartharius K, Frech K, Grote K, Klocke B, Haltmeier M, Klingenhoff A, Frisch M, Bayerlein M, Werner T:MatInspector and beyond: promoter analysis based on transcription factor binding sites.Bioinformatics 2005, 21(13):2933-42