IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PKC activity regulates motility and is involved in the maintenance of plasma membrane organization in boar spermatozoa
Autor/es:
TEIJEIRO JM; BRAGADO MARIA J; MARINI PATRICIA; GARCÍA-MARÍN LUIS J
Lugar:
Sevilla
Reunión:
Congreso; 37th International Congress Federation of European Biochemical Societies (FEBS) and 22 th congress. International Union of Biochemistry and Molecular Biology; 2012
Resumen:
Protein kinase C (PKC) is a key regulatory enzyme in signal transduction mechanisms. Diacylglycerol and phorbol esters, which stimulate PKC activity, enhance the onset of the early stages of the acrosome reaction in mouse, human, ram and bovine spermatozoa. PKC activator phorbol-12-myristate-13-acetate (PMA) stimulates boar sperm protein Ser phosphorylation in specific PKC recognized motifs while it does not affect acrosome reaction. Our aim is to study the effect of PMA on boar sperm motility and plasma membrane function under non-capacitating (TBM) or capacitating (TCM) conditions. Spermatozoa viability, plasma membrane scrambling (PMS) and the acrosome functional status were analyzed by flow cytometry. Motility was analyzed by the ISAS program. PMA treatment increases PMS from 13.6±1.2% to 24.5±3.1% in TBM and from 28.2±4.4% to 43.1±5.4% in TCM, this effect was lower compared with a cAMP analogue 8Br-cAMP. This PMA effect in PMS is blocked by H89, a protein kinase A inhibitor, indicating a crosstalk between PKA and PKC pathways. Moreover, PMA induces head-to-head spermatozoa agglutination with intense flagellar beating. PMA-induced PKC activity decreases the percentage of rapid spermatozoa (average path velocity>80μm/s) from 64% to 47% (TBM) and from 61% to 35% (TCM). Our data show that PKC activity in boar spermatozoa reduces the percentage of rapid spermatozoa and increases plasma membrane scrambling, which could be responsible for the head-to-head agglutination Supported by Grants: MICINN AGL2010-15188 and GR10156-JUEX